柠条缩合单宁提取工艺优化

Extraction Process Optimization of Condensed Tannin from Caragana korshinskii

[目的]确定柠条缩合单宁最佳提取工艺。[方法]以从内蒙古四子王旗采集的柠条为试验材料,利用超声辅助溶剂浸提法提取缩合单宁,利用磷钨钼酸显色法测定缩合单宁含量;应用单因素试验研究提取温度、提取时间、液料比、丙酮体积分数对柠条中缩合单宁提取量的影响。[结果]随提取温度的增加,柠条缩合单宁提取量呈先升高后降低的趋势,当提取温度为60℃时,提取量最大,为34.24 mg/g;随提取时间的增加,柠条缩合单宁提取量呈先升高后降低的趋势,当提取时间为80 min时,提取量最大,为26.38 mg/g;随液料比的增加,柠条缩合单宁提取量呈先升高后降低的趋势,当液料比为30∶1时,提取量最大,为25.34 mg/g;随丙酮体积分数的增加,柠条缩合单宁提取量呈先升高后降低的趋势,当丙酮体积分数为70%时,提取量最大,为30.61 mg/g。[结论]提取柠条缩合单宁的最适温度为60℃,最适时间为80 min,最适液料比为30∶1,最适丙酮体积分数为70%。

[Objective]The purpose of this study was to determine the optimized extraction process of condensed tannin from Caragana korshinskii.[Method]The Caragana korshinskii collected from Siziwang Banner of Inner Mongolia was used as experimental material.The condensed tannin was extracted from Caragana korshinskii by using ultrasonic assisted solvent extraction method,and the content of condensed tannin was determined by using phosphomolybdic and phosphotungstic acids chromogenic method.The effects of extraction temperature,extraction time,liquid to material ratio and acetone volume fraction on the yield of condensed tannin from Caragana korshinskii were assessed by single factor experiment.[Result]As the increase of extraction temperature,the yield of condensed tannin from Caragana korshinskii increased first and then decreased.When the extraction temperature was 60℃,the yield of condensed tannin was the highest,which was 34.24 mg/g.As the extension of extraction time,the yield of condensed tannin from Caragana korshinskii increased first and then decreased.When the extraction time was 80 min,the yield of condensed tannin was the highest,which was 26.38 mg/g.As the increase of liquid to material ratio,the yield of condensed tannin from Caragana korshinskii increased first and then decreased.When the liquid to material ratio was 30∶1,the yield of condensed tannin was the highest,which was 25.34 mg/g.As the increase of acetone volume fraction,the yield of condensed tannin from Caragana korshinskii increased first and then decreased.When the acetone volume fraction was 70%,the yield of condensed tannin was the highest,which was 30.61 mg/g.[Conclusion]The optimum extraction temperature,extraction time,liquid to material ratio and acetone volume fraction was 60℃,80 min,30∶1 and 70%,respectively.