AQP4、FASN、EphA2在脑膜瘤组织中的表达及相关性研究

Study on the expression and correlation of AQP4,FASN and EphA2 in meningioma tissue

目的分析水通道蛋白4(aquaporin 4,AQP4)、脂肪酸合成酶(fatty acid synthase,FASN)、上皮细胞激酶A2(epithelial kinase A2,EphA2)在脑膜瘤组织中的表达及相关性。方法选取脑膜瘤患者43例,对患者行手术切其脑膜瘤组织,分为脑膜瘤组,同时选取因外伤手术而切除的脑膜组织43例。实时荧光定量聚合酶链反应法检测AQP4相对表达量,免疫组织化学法检测FASN、EphA2相对表达量,对AQP4、FASN、EphA2在脑膜瘤组织中的表达及相关性进行分析。结果相比正常脑膜组织,脑膜瘤组织中的AQP4、FASN、EphA2表达升高(P<0.05);AQP4、FASN、EphA2相对表达量与脑膜瘤分级、瘤周水肿、骨侵犯具有相关性,差异有统计学意义(P<0.05)。其中Ⅱ级、Ⅲ级、有瘤周水肿、存在骨侵犯的脑膜瘤组织中的AQP4、FASN、EphA2相对表达量均显著升高(P<0.05)。相关性分析显示,脑膜瘤组织的AQP4、FASN表达呈正相关(r=0.506,P<0.001);FASN、EphA2表达呈正相关(r=0.528,P<0.001);AQP4 EphA2表达呈正相关(r=0.507,P<0.001)。ROC曲线分析显示,与AQP4、FASN、EphA2单项诊断相比,三项联合对脑膜瘤的诊断价值最高(P=0.001)。结论AQP4、FASN、EphA2在脑膜瘤中的相对表达量升高,且与患者病理特征相关,临床可依照其相对表达量的高低来对疾病进行评估。

Objective To analyze the expression and correlation of aquaporin 4(AQP4),fatty acid synthase(FASN),and epithelial kinase A2(EphA2)in meningioma tissue.Methods A total of 43 meningioma patients were selected,their meningioma tissue was surgically removed and enrolled as meningioma group,and 43 meningioma tissues removed by trauma surgery were selected.Relative expression of AQP4 was measured by quantitative real-time polymerase chain reaction(PCR),and relative expression of FASN and EphA2 was detected by immunohistochemistry.The expression and correlation of AQP4,FASN,and EphA2 in meningioma tissues were analyzed.Results Compared with normal meningeal tissues,the expressions of AQP4,FASN,and EphA2 in meningioma tissues were increased(P<0.05).The relative expressions of AQP4,FASN,and EphA2 were correlated with meningioma grade,peritumoral edema,and bone invasion(P<0.05).Among them,the relative expressions of AQP4,FASN,and EphA2 in patients with gradeⅡ,gradeⅢmeningiomas,peritumoral edema,and bone invasion were significantly increased(P<0.05).Correlation analysis showed that the expression of AQP4 and FASN in meningioma tissues was positively correlated(r=0.506,P<0.001);The expression of FASN and EphA2 was positively correlated(r=0.528,P<0.001);The expression of AQP4 and EphA2 was positively correlated(r=0.507,P<0.001).ROC curve analysis showed that compared with the single diagnosis of AQP4,FASN,and EphA2,the combination of the three had the highest diagnostic value for meningioma(P=0.001).Conclusion The relative expression of AQP4,FASN,and EphA2 in meningioma is increased,and is related to the pathological characteristics of the patient.The clinical evaluation of the disease can be made based on the relative expression.