眉山市7135例孕早期孕妇红细胞叶酸及血清维生素B12检测结果分析及正常参考值建立

Analysis of erythrocyte folic acid and serum vitamin B12 levels of 7135 pregnant women during the first trimester of pregnancy in Meishan city and the establishment of normal reference values

目的:了解四川省眉山地区孕早期孕妇红细胞叶酸和维生素B12水平,并建立正常参考范围。方法:对2018年10月-2021年2月在本院门诊产前检查,年龄18~45岁、孕8~12周孕妇,采用Roche cobas e411全自动电化学发光免疫分析仪对7135例红细胞叶酸样本和6296例血清维生素B12样本进行检测,统计分析建立参考值。结果:本次研究的孕妇红细胞叶酸参考范围为1045.22(606.11~1492.69)ng/ml,血清维生素B12参考范围为499.60(201.0~927.3)pg/ml。随着孕妇年龄增长红细胞叶酸浓度增加(P<0.001),仅7例(0.098%)低于预防新生儿神经管缺陷推荐的最适浓度(400 ng/ml);血清维生素B12水平<191 ng/ml的孕妇占2.3%(146/6296),集中在18~35岁,不同年龄段孕妇血清维生素B12水平未见差异,维生素B12缺乏随孕妇年龄增长而增加。结论:建立了眉山地区孕早期孕妇红细胞叶酸和血清维生素B12的参考范围,可为本地区孕期管理提供参考依据。

Objective: To understand the levels of erythrocyte folic acid and serum vitamin B12 of 7135 pregnant women during the first trimester of pregnancy in Meishan city, and to establish the normal reference values of erythrocyte folic acid and serum vitamin B12 levels. Methods: 7135 samples of erythrocyte folic acid and 6296 samples of serum vitamin B12 of pregnant women aged 18-45 years old and during 8-12 gestational weeks from October 2018 to February 2021 were measured by Roche Cobas e411 automatic electrochemiluminescence immunoanalyzer. The measurement results were analyzed statistically and the reference range of erythrocyte folate and serum vitamin B12 levels were established. Results: The reference ranges of erythrocyte folic acid and serum vitamin B12 levels of the pregnant women were 1045.22(606.11-1492.69)ng/mL and 499.60(201.0-927.3) pg/ml. The erythrocyte folic acid level of the women had increased with the maternal age increasing(P<0.001). Only 7 women(0.098%) had the erythrocyte folic acid level lower than that(400 ng/ml) of recommended level for preventing neonatal neural tube defects. The proportion of the pregnant women with serum vitamin B12 level <191 ng/ml was 2.3%(146/6296), which mainly in 18-35 years old. There was no significant difference in the serum vitamin B12 level among the pregnant women with different ages, and the proportion of the vitamin B12 deficiency of the women had increased with their age increasing. Conclusion: The establishment of the normal reference range of erythrocyte folic acid and serum vitamin B12 of the pregnant women during the first trimester of pregnancy in Meishan can provide theoretical evidences for pregnant management in location.