子宫内膜异位症不孕患者异位内膜组织PAQR3、CAMS、HIF-1α表达及与生育指数关系

Correlation between the expression levels of adipoQ receptor 3, cell adhesion molecules, and hypoxia-inducible factor-1αof infertility women with endometriosis and their fertility index

目的:探究孕激素和脂联素分子受体3(PAQR3)、细胞黏附分子(CAMs)、缺氧诱导因子-1α(HIF-1α)在子宫内膜异位症(EMs)不孕中的表达及可能关系。方法:选取2019年4月-2020年4月本院收治的EMs致不孕的异位内膜42例(异位内膜组),EMs在位内膜30例(在位内膜组),正常子宫内膜20例(正常内膜组),检测不同内膜组织标本中PAQR3、CAMs、HIF-1α表达并分析与EMs生育指数关系。结果:异位内膜组、在位内膜组、正常内膜组PAQR3阳性表达率分别为33.3%、66.7%、85.0%,CD44阳性表达率分别为88.1%、46.7%、20.0%,HIF-1α蛋白表达阳性率分别为83.3%、53.3%、0,异位内膜组阳性率均高于另外两组(P<0.05),但异位内膜组不同分期患者PAQR3、CD44、HIF-1α阳性表达率无差异(P>0.05)。PAQR3表达阳性患者EFI评分均高于表达阴性患者,CD44、HIF-1α表达阳性患者EFI评分均低于表达阴性患者,且PAQR3与EFI评分呈正相关,CD44、HIF-1α均与EFI评分呈负相关(均P<0.05)。结论:EMs合并不孕患者异位内膜组织中PAQR3、CAMs、HIF-1α表达异常,提示三者可能促进EMs的发生,进而导致患者不孕。

Objective: To explore the expression of progestin and adipoQ receptor 3(PAQR3), cell adhesion molecules(CAMs), and hypoxia-inducible factor-1α(HIF-1α) of infertility women with endometriosis(EMs), and to study their possible correlation. Methods: 42 samples of ectopic endometrium of infertility women with Ems were selected in group A, 30 samples of eutopic endometrium of infertility women with EMs were selected in group B, and 20 samples of normal endometrium were selected in group C. The levels of PAQR3, CAMs, and HIF-1α in different endometrial tissues were detected, and the correlation between which and their endometrial fertility index(EFI) was analyzed. Results: The positive expression rates of PAQR3 in group A, B and C were 33.3%, 66.7% and 85.0%, respectively, the positive expression rates of CD44 in group A, B, and C were 88.1%, 46.7%, and 20.0%, respectively, the positive expression rates of HIF-1α protein in group A, B, and C were 83.3%, 53.3%, and 0, respectively. The positive rates of PAQR3, CD44, and HIF-1α protein in group A were significantly higher than those in group B and in group C(P<0.05), but in group A, there were no significant differences in the positive rates of PAQR3, CD44, and HIF-1αof the women with different EMs stages(P>0.05). The EFI score of the women with positive PAQR3 was significantly higher than that of the women with negative PAQR3, but the EFI scores of the women with positive CD44 and HIF-1α were significantly lower than those of the women with negative CD44 and HIF-1α. The PAQR3 level of the women was positively correlated with their EFI score, while the CD44 and HIF-1α levels of the women were negatively correlated with their EFI scores(all P<0.05). Conclusion: The levels of PAQR3, CAMs, and HIF-1αof infertility women with EMs are all abnormal,which suggest that the levels of PAQR3,CAMs,and HIF-1αmay promote the occurrence and development of Ems of the women,thus leading to their infertility.