表面等离子体共振传感器生物识别分子的固定化方法

Immobilization Methods of Bioreceptor for Surface Plasma Resonance Sensors

表面等离子体共振具有无需标记样品和实时检测等优点,广泛应用于药物筛选以及生物、药物、食品等领域的检测。构建合适的生物传感界面是提高检测灵敏度和选择性的重要途径。该文介绍了利用化学偶联生物识别分子的非定向固定方法,以及利用化学键合或生物分子的特异性反应定向固定生物识别分子的方法,比较了两种固定方法的优缺点,并讨论了未来的发展趋势。

Surface plasma resonance is widely used for drug screening,and the analysis in biological,pharmaceutical,food and other fields due to its advantages of no labeling and real-time detection.Constructing a suitable biosensing interface is an important way to improve the detection sensitivity and selectivity of biosensors.This review describes the non-oriented immobilization methods using chemically coupled bioreceptor and the oriented immobilization methods that utilize specific properties of chemical bonding or biomolecules.The advantages and disadvantages of each immobilization method are summarized,and the future development is also discussed.