玉米ZmPRR1-1基因启动子的克隆及序列分析

Cloning and Sequence Analysis of ZmPRR1-1 Gene Promoter in Maize

克隆玉米伪应答调控基因ZmPRR1-1启动子并对其序列进行分析,以期为深入研究ZmPRR1-1基因的调控机制及基因功能提供参考。以玉米黄早4的叶片为供试材料,参考已公布玉米自交系CML247基因组中Zm-PRR1-1启动子的序列信息设计引物,克隆ZmPRR1-1基因的启动子,并利用生物信息学对启动子中的核心区域、顺式作用元件、转录因子结合位点和CpG岛进行预测分析。克隆得到长度约2600 bp的ZmPRR1-1启动子,预测ZmPRR1-1启动子可能存在2个核心启动子区域,除了存在核心元件TATA-box和启动子增强元件CAAT-box外,还存在光响应、激素应答、胁迫响应等顺式作用元件。转录因子结合位点分析表明,ZmPRR1-1启动子区中预测包含ERF在内的6种转录因子家族,此外在启动子区域中预测存在4个符合限定条件的CpG岛区域。玉米ZmPRR1-1启动子区域存在丰富的顺式作用元件和转录因子结合位点,暗示ZmPRR1-1可能受到外界多种类型调控因子的调控并启动转录起始,且在多种调控网络中发挥功能。

The promoter of maize pseudo response regulatory gene ZmPRR1-1 was cloned and its sequence was analyzed in order to provide reference for further study on the regulatory mechanism and gene function of ZmPRR1-1.Using the leaves of maize Huangzao4 as test materials,primers were designed according to the published sequence information of ZmPRR1-1 promoter in the genome of maize inbred line CML247,the promoter of ZmPRR1-1 was cloned,and the core region,cis acting element,transcription factor binding site and CpG island in the promoter were predicted and analyzed by bioinformatics.The ZmPRR1-1 promoter with a length of about 2600 bp was cloned.It was predicted that there might be two core promoter regions in the ZmPRR1-1 promoter.Except the necessary promoter core element TATA box and promoter enhancement element CAAT box,there were also cis acting elements such as light,hormoneand stress response,etc.The analysis of transcription factor binding sites showed that six transcription factor families including ERF were predicted in the promoter region of ZmPRR1-1.In addition,four CpG island regions meeting the limited conditions were predicted in the promoter region.There were abundant cis acting elements and transcription factor binding sites in the promoter region of maize ZmPRR1-1,suggesting that ZmPRR1-1 may be regulated by various types of external regulatory factors and start transcription initiation,and play a role in a variety of regulatory networks.