茯苓HPLC指纹图谱的建立及三萜酸含量测定

Establishment of HPLC fingerprint of Poria cocos and determination of triterpene acid content

采用HydroBondPS-C_(18)色谱柱(4.6 mm×250.0 mm,5μm),以乙腈和0.2%甲酸水为流动相,进行梯度洗脱,流速为1.0 mL/min,柱温为30℃,检测波长为203、242 nm。结果显示,茯苓新酸A、茯苓酸、松苓新酸的线性范围分别为1.82~10.94μg、1.90~11.38μg、2.21~13.26μg,相关系数r=0.9999,说明两变量之间具有较好的相关性;平均加样回收率分别为99.32%、99.85%、99.01%,建立了茯苓(Poria cocos)的指纹图谱;11个地区茯苓的11个共有峰,其中,四川省西昌市茯苓新酸A、茯苓酸、松苓新酸的含量均最高,分别为4.21、4.39、4.06 mg/g。说明该方法稳定性好,重复性高,适用于茯苓指纹图谱的建立和三萜酸含量测定。

HydroBondPS-C_(18) Column(4.6 mm×250.0 mm,5μm)was used for gradient elution with acetonitrile and 0.2%formic acid water as the mobile phase.The flow rate was 1.0 mL/min,the column temperature was 30℃,and the detection wavelengths were 203 and 242 nm.The results showed that the linear ranges of pachymaric acid A,pachymaric acid and pineosinic acid were 1.82~10.94μg,1.90~11.38μg,2.21~13.26μg,respectively.The correlation coefficient was 0.9999,indicating that there was a good correlation between the two variables.The recovery rates were 99.32%,99.85%and 99.01%,respectively,and the fingerprint of Poria cocos was established.There were 11 common peaks of Poria cocos in 11 regions.Among them,the contents of pachymaric acid A,pachymaric acid and pineosinic acid in Xichang City of Sichuan Province were the highest,which were 4.21,4.39 and 4.06 mg/g,respectively.This method has good stability and high repeatability,which is suitable for the establishment of Poria cocos fingerprint and the determi⁃nation of triterpenoid acid content.