欧月红宝石冰组织培养快繁技术研究

Research on tissue culture and rapid propagation technology of European rose ruby ice

以欧月(Rosa chinensis Jacq.)品种红宝石冰为试验材料,取其半木质化的中部茎段作为外植体,成功建立了组织培养技术体系。结果表明,外植体最佳的灭菌方式是采用0.1%HgCl_(2)灭菌8 min;初代培养阶段最佳培养基配方为MS+0.2 mg/L NAA+2.0 mg/L 6-BA,继代增殖培养阶段最佳培养基配方为MS+0.2 mg/L NAA+2.5 mg/L 6-BA,生根培养阶段最佳培养基配方为1/2 MS+0.2 mg/L NAA;移栽的最佳基质为珍珠岩+草炭+蛭石,体积比为1∶1∶1。

The semi-lignified middle stem segments of European rose(Rosa chinensis Jacq.)variety ruby ice were used as explants,and the tissue culture system was successfully established.The results showed that the best sterilization method for explants was 0.1%HgCl_(2) for 8 min;the optimal medium formula in the primary culture stage was MS+0.2 mg/L NAA+2.0 mg/L 6-BA,the optimal medium formula in the subculture proliferation stage was MS+0.2 mg/L NAA+2.5 mg/L 6-BA,and the optimal medium formula in the rooting culture stage was 1/2 MS+0.2 mg/L NAA.The best transplanting substrate was perlite+peat+vermiculite,and the volume ratio was 1∶1∶1.