3D回旋仪模拟微重力效应对小鼠骨骼和代谢组学的影响

Effects of microgravity on skeletal and metabolomics of mice simulated by 3D clinostat

目的3D回旋仪被广泛的应用于植物、细胞和线虫模拟微重力效应的研究中。但是尚未建立相关的啮齿类动物实验模型并探讨其作用机制。方法将30只8周龄的雄性C57BL/J小鼠随机分成3组:独立通风笼组(MC组,n=6),生存盒组(SB组,n=12),3D回旋仪组(CS组,n=12)。实验结束后,使用Micro-CT检测小鼠左股骨丢失情况。采用代谢组学检测血清代谢物变化。结果3D回旋仪处理后,CS组小鼠呈现出骨小梁数量减少(P<0.05)和骨小梁间距增加(P<0.5)的特征。在代谢组学中发现CS组有86种代谢物具有显著性差异。这些差异代谢物主要参与了蛋白质的消化吸收和矿物质吸收等代谢通路。结论3D回旋仪模拟微重力效应引起了C57BL/J小鼠骨丢失和代谢物及代谢通路改变。

Objective Three-dimensional(3D)clinostat has been widely used to simulate the effect of microgravity effect on plants,cells,and nematodes.Although its mechanism in models other than rodents has been discussed,no rodent model has been established and discussed mechanism.Methods Thirty 8-week-old male C57BL/J mice were randomly divided into three groups:independent ventilation cage group(MC,n=6),survival box group(SB,n=12),and 3D clinostat group(CS,n=12).After the study,loss of the left femur was detected using Micro-CT and serum metabolite changes were tested using metabolomics.Results After 3D clinostat treatment,the trabecular number decreased(P<0.05)and trabeculae spacing increased(P<0.5)in the CS group.Eighty-six metabolites in the CS group exhibited significant differences in metabolomics.These differential metabolites were mainly involved in protein digestion and absorption,and mineral absorption.Conclusions 3D clinostat microgravity induced bone loss and changes in metabolites and metabolic pathways in C57BL/J mice.