白蜡虫脂酰辅酶A还原酶基因家族鉴定及序列分析

Identification and Sequence Analyses of Fatty acyl-CoA Reductase Gene Family of the Chinese White Wax Scale Insect(Ericerus pela)

【目的】对白蜡虫脂酰辅酶A还原酶(fatty acyl-CoA reductase,far)基因家族进行鉴定和序列分析。【方法】以泌蜡昆虫白蜡虫作为材料,利用生物信息学分析方法对白蜡虫far基因家族进行鉴定。【结果】在白蜡虫基因组中共鉴定到30个far候选基因,其中22个FAR结构域包含完整的1个NAD(P)H结合结构域和1个FAR C结构域。基序分析表明:多数白蜡虫FAR含有13个保守基序。白蜡虫far基因有6~11个外显子、5~10个内含子。白蜡虫FAR系统进化分析表明:白蜡虫FAR成员聚为2类。对不同物种FAR进行的系统进化分析表明:FAR聚类主要与物种亲缘关系有关,与功能的相关性不大。共线性分析与氨基酸相似性分析显示:亲缘关系相近的物种FAR相似性更高,一般同物种内FAR相似性最高。【结论】本研究有助于了解白蜡虫far基因家族的进化,为深入研究白蜡虫far基因家族参与不同的功能奠定基础。

[Purpose]To identify the fatty acyl-CoA reductase(far)gene family and to analyze the gene sequences.[Method]The far gene family of the Chinses white wax scale insect(Ericerus pela)was identified and analyzed by bioinformatics methods.[Results]There were a total of 30 candidate far genes were identified,and 22 genes of them had a relatively conserved structure,an NAD(P)H binding domain and a FAR C domain.Motif analysis showed that there were 13 conserved motifs in these FAR.The far genes of E.pela had 6-11exons and 5-10 introns.The phylogenetic ana-lysis showed that FAR of E.pela were divided into two groups.The phylogenetic tree based on FAR of different species revealed FAR cluster groups closely related to species rather than their functions.Synteny analysis and amino acid similarity analysis showed that FAR generally had the highest simil-arity within the same species.[Conclusion]This study was helpful to understand the evolution of far gene family in E.pela and provided a basis for further research on the molecular functions of far gene family of E.pela.