细梗香草药材HPLC指纹图谱研究及质量评价

Establishment of HPLC Fingerprint of Lysimachia Capillipes Hemsl.and Its Quality Evaluation

目的 通过HPLC建立细梗香草药材的指纹图谱,并对其进行聚类分析和主成分分析。方法 利用《中药色谱指纹图谱相似度评价系统》(2012版)对12批次细梗香草药材进行相似度评价,并采用SPSS 21.0软件对其进行聚类分析和主成分分析。结果 建立了细梗香草药材HPLC指纹图谱,确定了12个共有峰,指认了其中5个色谱峰;12批药材细梗香草相似度为0.949~0.997,表明它们之间的一致性较好。12批细梗香草可以聚为两大类,其中S7、S8聚为一类,其余聚为一类。经主成分分析,主成分1~3是影响细梗香草质量评价的主要因子,3个主成分累计方差贡献率为96.93%。结论 所建立的细梗香草的HPLC指纹图谱,以及聚类分析和主成分分析结果可为细梗香草药材的质量评价及进一步开发利用提供参考。

OBJECTIVE To establish fingerprint of Lysimachia capillipes Hemsl.by HPLC,and to evaluate the quality of Lysimachia capillipes Hemsl.by cluster analysis and principal component analysis.METHODS Used the “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation” software(2012 edition),12 batches of Lysimachia capillipes Hemsl.were evaluated for similarity,and SPSS 21.0 statistical software was used for system cluster analysis and principal component analysis.RESULTS The established HPLC fingerprint of Lysimachia capillipes Hemsl.defined 12 commons peaks and identified 5common peaks.The similarity of 12 batches of samples was 0.949-0.997,indicating the similarity of 12 batchs of samples were good.Twelve batches of samples were clustered into 2 categories;S7 and S8 were clustered into one category;the rest were custered into the other category.As principal component analysis,principal component 1-3 were main influential factors of medcicinal material quality;accumulative variance contribution rate of them was 96.93%.CONCLUSION The established HPLC fingerprint of Lysimachia capillipes Hemsl.,and the results of cluster analysis and principal component analysis can provide reference for quality evaluation of Lysimachia capillipes Hemsl.and its exploitation and utilization.