伞裙追寄蝇滞育关联基因和滞育关联代谢物的联合分析

Combined analysis of diapause-associated genes and diapause-associated metabolites in Exorista civilis

为探索伞裙追寄蝇蛹滞育调控的分子机制,本文对伞裙追寄蝇Exorista civilis滞育蛹和非滞育蛹进行转录组测序以及代谢组检测,鉴定其关键的滞育关联基因(diapause-associated genes,DEGs)和滞育关联代谢物(diapasuse-associated metabolites,DEMs)。本研究基于高通量测序以及液质联用技术,通过筛选,在转录组中获得了差异表达基因7513个,在代谢组中,获得差异代谢物501个,其中氨基酸占比最多。将所有差异表达基因与差异表达代谢物同时向KEGG映射,获得两者共同的pathway信息,明确差异表达基因与差异代谢物共同参与的主要生化途径和信号转导途径。本研究通过转录组和代谢组的联合分析,在正、负离子模式下,差异表达基因与差异代谢物共同富集到70条通路。在负离子模式下,滞育关联因子主要参与氨基酸代谢和神经系统;在正离子模式下,滞育关联因子主要参与消化系统和信号转导途径。本研究重点分析了柠檬酸循环、cAMP信号通路、氨酰-tRNA的生物合成,将为进一步深入研究伞裙追寄蝇滞育调控的分子机制奠定理论基础。

To clarify the molecular mechanisms underlying the regulation of diapause regulation in the pupae of Exorista civilis,we performed transcriptome sequencing and metabolomic assays to identify key diapause-associated genes(DEGs)and diapause-associated metabolites(DEMs)in those diapause and non-diapause pupae.In this study,7513 differentially expressed genes in the transcriptome and 501 differential metabolites in the metabolome were obtained based on high-throughput sequencing and liquid chromatography mass spectrometry,with the largest proportion of amino acids.All the differentially expressed genes and differentially expressed metabolites were mapped to KEGG database simultaneously to obtain their common pathway information,in order to clarify the main biochemical pathways and signal transduction pathways they were jointly involved.In this study,the differentially expressed genes were enriched to 70 pathways together with differential metabolites in positive and negative ion modes by combined transcriptome and metabolome analysis.In the negative ion mode,the diapause-associated factors were mainly engaged in amino acid metabolism and nervous system;in the positive ion mode,the diapause-associated factors were mainly involved in digestive system and signal transduction pathway.This study focused on the analysis of citric acid cycle,cAMP signaling pathway and aminoacyl-tRNA biosynthesis,which would provide the theoretical foundation for the further study of the molecular mechanism of diapausing regulation in E.civilis.