氧化苦参碱对非酒精性脂肪性肝病大鼠肝脏保护作用研究

Protective effect of oxymatrine on liver steatosis in rats with high-fat-induced non-alcoholic fatty liver diseases

目的观察氧化苦参碱(OMT)干预对非酒精性脂肪性肝病(NAFLD)大鼠肝脏的保护作用。方法随机将40只SD大鼠分为对照组10只、模型组10只、OMT干预组10只和辛伐他汀干预组10只,采用高脂饮食饲养建立NAFLD模型,自第9 w开始分别给予OMT或辛伐他汀灌胃至16 w。采用ELISA法检测血清白细胞介素-1β(IL-1β)、IL-6、IL-10和肿瘤坏死因子-α(TNF-α)水平。取肝组织匀浆,检测超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)和丙二醛(MDA)水平。结果OMT干预组大鼠体质量和肝质量分别为(610.3±9.4)g和(11.6±0.7)g,显著低于模型组【分别为(631.8±13.9)g和(13.9±0.6)g,P<0.05】;血清ALT和AST水平分别为(78.9±7.0)U/L和(120.4±11.3)U/L,显著低于模型组【分别为(96.7±11.4)U/L和(183.1±25.9)U/L,P<0.05】;血清TC、TG和LDL水平分别为(2.0±0.2)mmo/L、(2.2±0.1)mmo/L和(1.0±0.1)mmo/L,显著低于模型组【分别为(2.4±0.2)mmo/L、(2.8±0.2)mmo/L和(1.2±0.2)mmo/L,P<0.05】;血清IL-1β、IL-6和TNF-α水平分别为(6.4±1.8)pg/ml、(63.7±8.5)pg/ml和(13.9±1.9)pg/ml,显著低于模型组【分别为(13.9±8.4)pg/ml、(149.8±12.0)pg/ml和(36.5±2.9)pg/ml,P<0.05】,而血清IL-10水平为(42.3±2.0)pg/ml,显著高于模型组【(18.9±1.9)pg/ml,P<0.05】;肝组织匀浆SOD和GSH水平分别为(22.3±2.1)μg/mg和(26.0±2.1)U/mg,显著高于模型组【分别为(17.5±1.9)μg/mg和(15.8±1.8)U/mg,P<0.05】,而MDA水平为(17.9±2.2)nmol/mg,显著低于模型组【(23.8±2.7)nmol/mg,P<0.05】。结论氧化苦参碱可有效保护NAFLD大鼠肝功能,降低血脂水平,可能与其抗炎和抗氧化应激作用有关。

Objective The aim of this experiment was to observe the protective effect of oxymatrine(OMT)on liver steatosis in rats with high-fat-induced non-alcoholic fatty liver diseases(NAFLD).Methods 40 SD rats were randomly divided into control,model,OMT–intervened and simvastatin-intervened groups,with 10 rats in each.The NAFLD model was established by high-fat diet feeding.Since 9^(th) week,the normal saline,OMT or simvastatin was given by gavage,and the management continued to 16^(th) week.Serum interleukin-1β(IL-1β),IL-6,IL-10 and tumor necrosis factor-α(TNF-α),and liver homogenate superoxide dismutase(SOD),reduced glutathione(GSH)and malondialdehyde(MDA)levels were detected.Results The body mass and liver mass in OMT-intervened group were(610.3±9.4)g and(11.6±0.7)g,significantly lower than[(631.8±13.9)g and(13.9±0.6)g,respectively,P<0.05]in the model;serum ALT and AST levels were(78.9±7.0)U/L and(120.4±11.3)U/L,significantly lower than[(96.7±11.4)U/L and(183.1±25.9)U/L,respectively,P<0.05]in the model;serum TC,TG and LDL levels were(2.0±0.2)mmo/L,(2.2±0.1)mmo/L and(1.0±0.1)mmo/L,significantly lower than[(2.4±0.2)mmo/L,(2.8±0.2)mmo/L and(1.2±0.2)mmo/L,respectively,P<0.05]in the model group;serum IL-1β,IL-6 and TNF-αlevels were(6.4±1.8)pg/ml,(63.7±8.5)pg/ml and(13.9±1.9)pg/ml,significantly lower than[(13.9±8.4)pg/ml,(149.8±12.0)pg/ml and(36.5±2.9)pg/ml,respectively,P<0.05],while serum IL-10 level was(42.3±2.0)pg/ml,significantly higher than[(18.9±1.9)pg/ml,P<0.05]in the model;the liver homogenate SOD and GSH levels were(22.3±2.1)μg/mg and(26.0±2.1)U/mg,significantly higher than[(17.5±1.9)μg/mg and(15.8±1.8)U/mg,respectively,P<0.05]while MDA level was(17.9±2.2)nmol/mg,significantly lower than[(23.8±2.7)nmol/mg,P<0.05]in the model.Conclusion Oxymatrine could protect the liver function of rats with NAFLD and reduce blood lipids levels,which might be related to its anti-inflammatory and anti-oxidative stress effects.