“cell-in-cell”对胃癌细胞生物学行为的影响

Effect of"cell in cell"on biological behavior of gastric cancer cells

目的探究胃癌肿瘤细胞与免疫细胞cell-in-cell的形成对胃癌细胞生物学行为的影响。方法胃癌细胞与单核淋巴细胞(PBMC)的共培养体系分为对照组、1∶1组和5∶1组。PBMC和BGC823细胞分别以1∶1和5∶1的接种到24孔板中(共5×10^(4)个)。通过吉姆萨染色检测“cell-in-cell”构建情况。分别检测各组细胞增殖、凋亡和侵袭能力。结果1∶1组的“cell-in-cell”构建率为(8.65±1.06)%。5∶1组的“cell-in-cell”构建率为(12.92±1.32)%,显著高于1∶1组(P<0.05)。3组细胞的生物学行为水平比较差异显著(P<0.05)。1∶1组的增殖和侵袭能力显著高于对照组,而凋亡率显著低于对照组(P<0.05)。5∶1组的增殖和侵袭能力显著高于1∶1组和对照组,而凋亡率显著低于1∶1组和对照组(P<0.05)。结论胃癌细胞与免疫细胞形成的“cell-in-cell”结构会促进胃癌细胞的增殖和侵袭并抑制凋亡。

Objective To explore the influence of the formation of gastric cancer tumor cells and immune cells cell-in-cell on the biological behavior of tumor cells.Methods The co-culture system of gastric cancer cells and mononuclear lymphocytes(PBMC)were divided into control group,1∶1 group and 5:1 group.PBMC and BGC823 cells were seeded into 24-well plates at 1∶1 and 5∶1,respectively(5×10^(4) cells in total).The construction of"cell-in-cell"was detected by Giemsa staining.Cell proliferation,apoptosis and invasion ability of each group were tested.Results The construction rate of"cell-in-cell"for the 1∶1 group was(8.65±1.06)%.The"cell-in-cell"construction rate of the 5∶1 group was(12.92±1.32)%,which was significantly higher than that of the 1∶1 group(P<0.05).The biological behavior levels of the three groups of cells were significantly different(P<0.05).The proliferation and invasion abilities of the 1∶1 group were significantly higher than those of the control group,while the apoptosis rate was significantly lower than that of the control group(P<0.05).The proliferation and invasion abilities of the 5∶1 group were significantly higher than those of the 1∶1 group and the control group,while the apoptosis rate was significantly lower than that of the 1∶1 group and the control group(P<0.05).Conclusion The"cell-in-cell"structure formed by gastric cancer cells and immune cells will promote the proliferation and invasion of gastric cancer cells and inhibit apoptosis.