马尾松叶片挥发物组成及其对松墨天牛有触角电生理反应的活性成分鉴定

Identification of Leaf Volatiles from Pinus massoniana and Antennal Electrophysiological Activity of Volatiles to Monochamus alternatus

为了明确马尾松Pinus massoniana叶片中吸引松墨天牛Monochamus alternatus的特定挥发性化合物,利用Y型嗅觉仪测试了马尾松挥发物对松墨天牛的趋性,应用GC-MS分析鉴定了马尾松叶片中挥发物的组成,并使用GC-EAD筛选了能引起松墨天牛触角电生理反应的活性化合物。结果表明:松墨天牛对马尾松挥发物具有较强的选择趋性;GC-MS分析表明马尾松叶片中共有22个化合物,其中萜烯类物质14种,醇类物质5种,羧酸类物质和酯类物质各1种,以及1个未鉴定的化合物。在上述挥发物中,β-蒎烯的相对含量最高,为(28.68±0.67)%,其次为α-蒎烯,为(21.03±5.08)%;α-蒎烯、β-蒎烯、β-水芹烯、β-石竹烯、D-吉玛烯以及乙酸龙脑酯6个化合物能引起松墨天牛雌雄成虫的触角电生理反应。

In order to identify the specific volatile compounds that attract the Japanese pine sawyer,Monochamus alternatus Hope, 1842(Coleoptera: Cerambycidae), in the leaves of the Masson’s pine, Pinus massoniana, the Y-type olfactometer was used to determine the attractive effect of volatile compounds of P.massoniana leaves and the composition in leaf volatiles of P. massoniana was analyzed and identified by GC-MS,as well as the active compounds that can cause the electrophysiological response of the antennae of Japanese pine sawyers were screened by GC-EAD. The results showed that the volatiles of Masson’s pine could significantly attract adults of Japanese pine sawyers. GC-MS results showed that there were 22 compounds in the Masson’s pine leaves, including 14 terpenoids, 5 alcohols, 1 carboxylic acid and 1 ester, as well as one unidentified compound. Among the leafy volatiles, the relative percentage of β-pinene was the highest(28.68 ± 0.67)%,followed by α-pinene(21.03 ± 5.08)%;Six compounds of α-Pinene, β-Pinene, β-Carylene, β-Dianthus caryophyllene, D-gemonene and bornyl acetate could elicit the antennal electrophysiological responses of male and female adults of the pine sawyer. These results will provide a reference for the chemical mechanism of M.alternatus location for the host plant and the improvement of the attractant against Japanese pine sawyers.