纸片型1-MCP处理对‘朝霞’水蜜桃抗病物质代谢的影响

Effects of paper-containing 1-methylcyclopropene treatment on the disease resistance metabolism of harvested ’Zhaoxia’ peach fruit

目的 研究纸片型1-甲基环丙烯(1-methylcyclopropene,1-MCP)处理对采后水蜜桃果实抗病物质代谢的影响。方法 ‘朝霞’水蜜桃果实采后通过0(对照组)和1.2μL/L纸片型1-MCP分别处理12 h,在(25±1)℃、85%相对湿度下贮藏,每2d取样,评价和测定水蜜桃果实感病指数、抗病相关物质(总酚、木质素)含量及抗病相关酶[过氧化物酶(peroxidase,POD)、多酚氧化酶(polyphenol oxidase,PPO)、几丁质酶(chitinase,CHI)、苯丙氨酸解氨酶(phenylalanine ammonia-lyase,PAL)和β-1,3-葡聚糖酶(β-1,3-glucanase,GLU)]活性。结果 与对照组水蜜桃果实相比,纸片型1-MCP处理能有效延缓水蜜桃果实采后感病指数的上升,维持较高的果实果肉木质素和总酚含量,保持较高的果肉POD、PPO、CHI、PAL和GLU活性。结论 纸片型1-MCP处理可升高水蜜桃果实采后果肉抗病相关酶的活性,加速果肉累积抗病物质,进一步提升水蜜桃果实的采后抗病能力,最终减慢果实采后发生病害及延长果实采后贮藏期。

Objective To study the effects of paper-containing 1-methylcyclopropene(1-MCP) treatment on the disease resistance metabolism of harvested peach fruit.Methods The fresh harvested ‘Zhaoxia’ peach fruit was treated by paper-containing 1-MCP at the dosages of 0(control group) and 1.2 μL/L for 12 h,then stored at(25±1)℃and relative humidity of 85%.During the storage,the fruit disease index,content of substances related to disease resistance,including total phenolics as well as lignin,and activities of disease resistance-related enzymes,such as peroxidase(POD),polyphenol oxidase(PPO),chitinase(CHI),phenylalanine ammonia-lyase(PAL) and β-1,3-glucanase(GLU) in pulp of peach fruit were assayed every 2 days.Results In contrast to control peach fruit,the paper-containing 1-MCP treatment might effectively delay the increase of fruit disease index of peach fruit during storage.In addition,the paper-containing 1-MCP treatment might maintain the higher content of lignin and total phenolics,and the higher activities of POD,PPO,CHI,PAL and GLU in pulp of peach fruit during storage.Conclusion The paper-containing 1-MCP treatment can improve the disease resistance,suppress the disease development and extend the storage-life of harvested peaches by enhancing the activities of disease resistance-related enzymes and accumulating the amounts of disease resistance-related substances.