市售生禽类产品分离大肠埃希氏菌药物敏感分析

Drug sensitivity analysis of Escherichia coli isolated from commercial poultry products

目的 分离和鉴定生禽类产品中的大肠埃希氏菌,并确定菌株的耐药情况。方法 从市场上随机抽取65份生禽类产品,采用选择性培养基分离并通过生化鉴定大肠埃希氏菌;分析未加工或不同的加工工艺生禽类产品中大肠埃希氏菌检出情况并通过抗生素最小抑制浓度掌握分离菌株药敏谱。结果 从65份样本中分离得到55株大肠埃希氏菌,检出率高达84.62%;其中未加工生鲜类为100.00%、冷却工艺为89.00%、冷冻工艺为70.00%。药敏结果显示,分离菌对亚胺培南(98.20%)、头孢西丁(92.70%)、头孢他啶(90.90%)、多粘菌素(90.90%)耐药率较高,对四环素最敏感(72.20%)。表型不同的大肠埃希氏菌分为典型菌株(45株)和非典型菌株(10株)药敏谱无显著性差异(P>0.05)。受试菌100%携带3种以上抗生素,有83.64%受试菌同时携带4种高耐药率的抗生素(亚胺培南、头孢西丁、头孢他啶、多粘菌素)。结论 市售生禽类产品中存在非常严重的多重耐药性大肠埃希氏菌污染。本研究对大肠埃希氏菌的药性分析,可以为禽类养殖企业规范用药提供理论参考。

Objective To isolate and identify Escherichia coli in raw poultry products and determine the drug resistance distribution of strains.Methods Sixty-five raw poultry products were randomly selected from the market,and Escherichia coli was isolated by selective medium and identified by biochemistry;the detection of Escherichia coli in raw poultry products without processing or different processing technologies was analyzed,and master the drug sensitivity spectrum of the isolated strains through the minimum inhibitory concentration of antibiotics.Results Fifty-five strains of Escherichia coli were isolated from 65 samples;the isolation rate was 84.62%;among them,100.00% were raw,89.00% were cooled and 70.00% were frozen.The drug sensitivity results showed that the resistance rates of isolates to imipenem(98.20%),cefoxitin(92.70%),ceftazidime(90.90%) and polymyxin(90.90%) were high,most sensitive to tetracycline(72.20%).There was no significant difference in drug sensitivity spectrum between typical strains(45 strains) and atypical strains(10 strains) with different phenotypes(P>0.05).100% of the tested bacteria carried more than 3 kinds of antibiotics,and 83.64% of the tested bacteria carried 4 kinds of antibiotics(imipenem,cefoxitin,ceftazidime,polymyxin) with high resistance rate at the same time.Conclusion There are very serious multi drug resistant Escherichia coli contamination in commercial raw poultry products.The drug analysis of Escherichia coli in this study can provide a theoretical reference for poultry breeding enterprises to standardize drug use.