不同花期蜂王浆主要成分和抗氧化活性分析

Analysis of the Main Components and Antioxidant Activity of Royal Jelly in Different Flowering Periods

为探究不同花期蜂王浆品质和抗氧化活性的差异,以同产地同蜂种同饲养条件下所生产的蜂王浆作为研究对象,分析不同花期蜂王浆的10-羟基-2-癸烯酸(10-HDA)、总蛋白、水分、总酚酸的含量变化,同时采用DPPH法和FRAP法分析比较其体外抗氧化能力。结果表明,不同花期之间蜂王浆的10-HDA含量存在显著差异(P<0.05),葡萄蜂王浆10-HDA含量最高达到了2.20%。总蛋白含量在13.58%~15.26%之间。蜂王浆的水分含量均≤67.5%,达到国标(GB 9697-2008)关于优等品蜂王浆水分含量的要求。与其他花期蜂王浆相比,荆条花期的总酚酸含量、DPPH自由基清除能力和总抗氧化能力均为最优,且不同花期蜂王浆之间的DPPH自由基清除率存在显著差异(P<0.05)。相关性分析表明,DPPH自由基清除能力和总酚酸以及总抗氧化能力之间均存在极显著正相关性(P<0.01)。

The aim of the present study was to explore the differences of quality and antioxidant activity of royal jelly in different flowering periods.The contents of 10-hydroxy-2-decenoic acid(10-HDA),total protein,moisture,and total phenolic acid in royal jelly from the same conditions were analyzed,and the in vitro antioxidant activities were also compared by DPPH method and FRAP method.The results showed that there were significant differences(P<0.05) in 10-HDA contents of royal jelly during different flowering periods,and the content of 10-HDA was the highest in grape royal jelly.The contents of total protein in royal jelly ranged from 13.58% to 15.26%.The contents of moisture in royal jelly were lower than 67.5%,which met the requirements of national standard(GB 9697-2008) regarding to the moisture content of premium royal jelly.Compared with other royal jelly at flowering periods,the total phenolic acid content,DPPH radical scavenging ability and total antioxidant capacity of Vitex royal jelly were the best.And the DPPH free radical scavenging rates of royal jelly at different flowering periods were significantly different(P<0.05).The correlation analysis results showed that the DPPH radical scavenging ability was significantly correlated with the total phenolic acid and the total antioxidant ability(P<0.01).