摘要
目的:研究略阳乌鸡汤对D-半乳糖致衰老小鼠不同组织的抗氧化作用,为略阳乌鸡的深加工和产业化开发提供理论支撑。方法:通过分析略阳乌鸡汤中矿物质元素和氨基酸含量,评价略阳乌鸡汤的主要营养价值。采用腹腔注射D-半乳糖构建衰老小鼠模型,用略阳乌鸡汤对衰老模型小鼠进行灌胃干预,连续干预10周。记录小鼠生长状态及体重变化,检测血清、睾丸、肝脏以及肾脏中超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力、总抗氧化能力(T-AOC)和丙二醛(MDA)含量。制备肝脏、肾脏、睾丸组织切片,通过HE染色观察组织病理学变化,通过SA-β-GAL染色分析不同处理组小鼠睾丸、肾脏和肝脏的衰老程度。结果:略阳乌鸡汤中未检出铅、砷、汞,钙、锌、硒含量分别为1040±2.59 mg/kg、3.25±0.65 mg/kg、8.72±0.53μg/kg;氨基酸总量1234 mg/100 g,其中必需氨基酸占25.5%,非必需氨基酸占74.5%,支链氨基酸占8.16%。与衰老模型组相比,略阳乌鸡汤干预组能显著(P<0.05)提高小鼠血清、睾丸、肝脏以及肾脏SOD活力、T-AOC、GSHPx活力,显著降低MDA含量(P<0.05)。HE染色和SA-β-GAL染色发现,略阳乌鸡汤能显著修复D-半乳糖对小鼠不同组织造成的损伤。结论:略阳乌鸡汤具有明显的抗氧化能力,可延缓衰老。
Objective:To study the antioxidant effect of Lüeyang black-bone chicken soup on different tissues of D-galactose-induced senescence mice,so as to provide theoretical support for the deep processing and industrial development of Lüeyang black-bone chicken.Methods:The main nutritional value of the soup was evaluated by analyzing the contents of mineral elements and amino acids.The aging mice model was established by intraperitoneal injection of D-galactose,and treated with Lüeyang black-bone chicken soup for 10 weeks.During the process,the changes of overall state and body weight of mice were recorded,and the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),total antioxidant capacity(T-AOC) and malondialdehyde(MDA) in serum,testis,liver and kidney were measured.Additionally,tissue sections such as liver,kidney and testis were prepared,and the histopathological changes were observed using HE staining,while the aging degree of testis,kidney and liver of mice in different groups were analyzed by SA-β-GAL staining.Results:Lead,arsenic and mercury were not detected in Lüeyang black-bone chicken soup,but the contents of calcium,zinc and selenium were 1040±2.59 mg/kg,3.25±0.65 mg/kg and 8.72±0.53 μg/kg.The total amount of amino acids was 1234 mg/100 g,of which essential amino acids accounted for 25.5%,non-essential amino acids accounted for 74.5% and branchain-chain amino acids accounted for 8.16%.Lüeyang black-bone chicken soup significantly increased the activity of SOD,T-AOC and GSH-Px in serum,testis,liver and kidney,while significantly decreased the level of MDA in mice(P<0.05).HE staining and SA-β-GAL staining revealed that Lüeyang black-bone chicken soup could significantly repair the damage of D-galactose to different tissues of mice.Conclusion:Lüeyang black-bone chicken soup has significant antioxidant capacity and can delay aging.
作者
刘茜
赵浩名
王令
王珊珊
路宏朝
袁国强
张涛
LIU Xi;ZHAO Haoming;WANG Ling;WANG Shanshan;LU Hongzhao;YUAN Guoqiang;ZHANG Tao(School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China;School of Medical Technology,Hanzhong Vocational and Technical College,Hanzhong 723000,China;Shaanxi Baiweiyuan Network Technology Co.,Ltd.,Lüeyang 724300,China)
出处
《食品工业科技》
CAS
北大核心
2022年第17期402-409,共8页
Science and Technology of Food Industry
基金
陕西省重点研发计划项目-农业领域(2020NY-001)。
关键词
略阳乌鸡汤
延缓衰老
抗氧化
Lüeyang black-bone chicken soup
anti-aging
antioxidant