银杏肽对高脂膳食诱导的高脂血症小鼠的降脂作用

Lipid-Lowering Effect of Ginkgo biloba Peptides on High-Fat Diet Induced Hyperlipidemia in Mice

基于高脂膳食诱导的高脂血症小鼠模型考察了银杏肽的辅助降脂作用。将小鼠随机分为正常组、高脂模型组、银杏肽低剂量(100 mg/kg)、银杏肽中剂量(200 mg/kg)、银杏肽高剂量组(400 mg/kg)和阳性对照组(10 mg/kg辛伐他汀),通过饲喂高脂饲料建立高脂血症小鼠模型。测定小鼠体质量、肝脏指数、摄食量、脂肪表观消化率;检测血清甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平;计算动脉粥样硬化指数(atherogenic index,AI)和冠心指数(coronary heart index,CHI);测定肝脏总抗氧化能力(total antioxidant capacity,T-AOC)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)、丙二醛(malondialdehyde,MDA)水平。结果表明,与高脂模型组相比,低、中、高剂量组银杏肽干预均显著降低了高脂血症小鼠体质量和肝脏指数(P<0.05),提高了脂肪表观消化率(P<0.05),对小鼠摄食量无显著性影响(P>0.05);降低了小鼠血清TC、TG、LDL-C(P<0.05)水平,升高了HDL-C水平(P<0.05);抑制了炎性因子IL-6和TNF-α水平;降低了小鼠AI和CHI(P<0.05);提高了肝脏T-AOC和T-SOD水平(P<0.05),降低了MDA水平(P<0.05)。与阳性对照组相比,高剂量组的肝脏指数、摄食量、脂肪表观消化率、TG、TC、HDL-C、CHI、IL-6、TNF-α均无显著性差异(P>0.05)。银杏肽对高脂膳食诱导的高脂血症小鼠具有辅助降脂作用,其作用机制与提高机体抗氧化能力和抑制促炎细胞因子表达有关。

This paper investigated the lipid-lowering effect of Ginkgo biloba peptides on high-fat diet induced hyperlipidemia in mice.Mice were randomly divided into six groups,including normal group,high-fat model group,Ginkgo biloba peptides low-dose(100 mg/kg),medium-dose(200 mg/kg),high-dose group(400 mg/kg) and positive control group(10 mg/kg simvastatin).A hyperlipidemia mice mode was established by feeding high-fat diet.Body weight,liver index,food intake,and apparent fat digestibility were measured.The kits were employed to determine the levels of the serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C) and high density liptein cholesterol(HDL-C),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),and the levels of the liver tissue total antioxidant capacity(T-AOC),total superoxide dismutase(T-SOD) and malondialdehyde(MDA).The atherogenic index(AI) and coronary heart index(CHI) were calculated.The results showed that the low,medium and high doses of Ginkgo biloba peptides significantly reduced the body weight and liver index(P<0.05),increased the apparent fat digestibility(P<0.05),had no significant effect on the food intake(P>0.05),down-regulated the TC,TG and LDL-C levels(P<0.05),up-regulated the HDL-C level,down-regulated the inflammatory cytokine IL-6 and TNF-α levels(P<0.05),reduced AI and CHI(P<0.05),promoted the T-AOC and T-SOD levels,and reduced the MDA level(P<0.05) as compared with the high-fat model group.No significant difference was found in liver index,food intake,apparent fat digestibility,TG,TC,HDL-C,CHI,IL-6,and TNF-α between the high-dose group and the positive control group(P>0.05).Ginkgo biloba peptides has an auxiliary lipid-lowering effect on high-fat diet induced hyperlipidemia in mice,and the underlying mechanisms is related to improving the body’s antioxidant capacity and inhibiting the expression of pro-inflammatory cytokines.