流式细胞术在嵌合抗原受体-T细胞免疫治疗相关实验室检测中应用的思考与展望

Thinking and prospect of the application of flow cytometry in laboratory testing of chimeric antigen receptor T cell immunotherapy

嵌合抗原受体(CAR)-T细胞免疫治疗是近年来肿瘤治疗领域最瞩目的成就之一,流式细胞术(FCM)在CAR-T细胞的设计构建、患者入组、CAR-T细胞产品的性能检测、治疗后疗效评价、免疫功能评价、复发机制研究与后续治疗方案的选择等各个环节都起到重要的作用。为了进一步推广与规范FCM在CAR-T细胞免疫治疗相关实验室检测中的应用,《中华检验医学杂志》组织了“流式细胞分析在CAR-T细胞免疫治疗中的应用和研究进展”专题报道,多篇文章分别涵盖FCM在CAR-T细胞免疫治疗中应用的多个方面,包括专家共识、继续教育、专家述评、微小残留病检测、免疫功能和细胞因子检测等,以期促进FCM在CAR-T细胞免疫治疗中应用的规范化,为临床应用提供更大的帮助。

Chimeric antigen receptor(CAR)-T cell immunotherapy is one of the most remarkable achievements in the field of cancer therapy for the past few years.Flow cytometry(FCM)plays an important role in the design and construction of CAR-T cells,patient enrollment,performance test of CAR-T products,evaluation of post-treatment efficacy and immune function,exploration of relapse mechanism,and selection of subsequent treatment.In order to further promote and standardize the application of FCM in laboratory testing related to CAR-T cell immunotherapy,the special issue on"Application and Research Progress of flow cytometry in CAR-T cell immunotherapy"were organized by The Chinese Journal of Laboratory Medicine.A number of articles related to this topic were published,which covered expert consensus,continuing education,expert review,detection of minimal residual disease,immune function,and cytokine detection.We intend to promote the standardization of FCM in CAR-T cell immunotherapy and provide greater assistance for clinical application.