基于深共晶溶剂溶解的虾青素及其乳液包埋体系制备与调控

Preparation and regulation of astaxanthin and its emulsion based on deep eutectic solvent system

虾青素(astaxanthin,AXT)特殊的化学结构导致其稳定性和溶解性差,极大地限制了其应用。采用麝草香酚和月桂酸为原料制备深共晶溶剂(deep eutectic solvent,DES)用于溶解AXT,再以AXT-DES溶液为油相、明胶和阿拉伯胶为水相,制备水包油型乳液,并探究乳液的形貌、稳定性、流变学特性和生物可及性。结果表明:当明胶/阿拉伯胶质量比为5、乳化剂质量分数为1.5%时,乳液稳定性较好,呈3.09μm的鲜红色球形乳滴,具有剪切变稀行为及类凝胶特性。AXT在DES中的溶解性良好,且在80℃时溶解度达到5.0%。储存20 d后,包埋组中AXT的保留率为83%,优于对照组的AXT保留率(78%)和已报道的包埋体系保留率(50.4%~79.25%)。包埋组的AXT生物可及性为83%,比对照组提升约37%。上述基于DES溶解AXT的乳液包埋体系为AXT的溶解与包埋提供了新策略。

Astaxanthin(AXT)has poor stability and solubility in water due to its special chemical structure,which extremely restricts its application.In this work,the deep eutectic solvent(DES)based on thymol and lauric acid was used to dissolve AXT.The oil-in-water(O/W)emulsions were prepared with AXT-DES solution as oil phase,gelatin and arabia gum as water phase.Meanwhile,the morphology,stability,rheological property and bioaccessibility of the prepared emulsions were investigated.The results demonstrated that when the mass ratio of gelatin/arabic gum was 5 and the mass fraction of emulsifier was 1.5%,emulsion stability was better with bright red spherical droplets of 3.09μm.The rheological result showed that emulsions had shear thinning behavior and gel-like properties.DES system had powerful ability for dissolving AXT,and the solubility of AXT in DES was 5.0%at 80℃.Moreover,the AXT retention rate in the encapsulated group was 83%after 20 d of storage,which was better than those in the control group(78%)and the reported studies(50.4%~79.25%).AXT bioaccessibility of the encapsulated group was 83%,which was 37%higher than that of the control group.It indicates that the AXT-loaded emulsion using DES system would provide a new strategy for AXT dissolution and encapsulation.