子宫颈癌患者组织中CK10、Ki-67表达及临床意义

Expression and Clinical Significance of CK10 and Ki-67 in Cervical Cancer Patients

目的:研究子宫颈癌患者组织中细胞角蛋白10(CK10)、Ki-67表达及临床意义。方法:回顾性分析2017年10月-2020年10月于九江市妇幼保健院完成子宫颈癌切除术的60例子宫颈癌患者临床资料。采集所有入组患者子宫颈癌组织及癌旁正常组织,以免疫组织化学法检测不同子宫颈组织CK10、Ki-67的表达情况。分析上述两项蛋白表达情况和子宫颈癌患者临床分期、淋巴结转移的关系。以受试者工作特征(ROC)曲线分析CK10、Ki-67评估子宫颈癌患者临床分期以及淋巴结转移的效能。结果:子宫颈癌组织CK10阳性率为18.33%,低于癌旁正常组织的80.00%,而Ki-67阳性率为88.33%,高于癌旁正常组织的11.67%,差异均有统计学意义(P<0.05)。临床国际妇产科联盟(FIGO)分期Ⅱ、Ⅲ期患者Ki-67阳性率为100%,均高于临床FIGO分期Ⅰ期患者,有淋巴结转移患者Ki-67阳性率为100%,高于无淋巴结转移患者,但差异均无统计学意义(P>0.05)。经ROC曲线分析可得,CK10与Ki-67联合检测评估子宫颈癌患者临床分期Ⅱ、Ⅲ期的曲线下面积、敏感度及特异度均高于上述两项指标单独检测,差异均有统计学意义(P<0.05)。结论:子宫颈癌患者组织中CK10呈异常低表达,而Ki-67呈异常高表达,联合检测其表达水平可能具有辅助评估子宫颈癌患者临床FIGO分期及淋巴结转移的作用。

Objective:To study the expression and clinical significance of CK10 and Ki-67 in cervical cancer patients.Method:The clinical data of 60 patients with cervical cancer who underwent cervical cancer resection in Maternal and Child Health Care Hospital of Jiujiang City from October 2017 to October 2020 were retrospectively analyzed.Cervical cancer tissues and normal adjacent tissues were collected from all enrolled patients,and the expressions of CK10 and Ki-67 in different cervical tissues were detected by immunohistochemistry.The relationship between the expression of the two proteins and clinical stage and lymph node metastasis of cervical cancer patients was analyzed.Receiver operating characteristic (ROC) curve analysis was used to determine the efficacy of CK10 and Ki-67 in evaluating clinical stage and lymph node metastasis of cervical cancer patients.Result:The positive rate of CK10 in cervical cancer tissues was 18.33%,lower than 80.00% of normal adjacent tissues,while the positive rate of Ki-67 was 88.33%,higher than 11.67% of normal adjacent tissues (P<0.05).The positive rate of Ki-67 in patients with clinical FIGO stage Ⅱ and Ⅲ was 100%,which were higher than that in patients with clinical FIGO stage Ⅰ,Ki-67 positive rate in patients with lymph node metastasis was 100%,higher than that in patients with no lymph node metastasis,but the differences were not statistically significant (P>0.05).ROC curve analysis showed that the area under curve,sensitivity and specificity of CK10 combined with Ki-67 in evaluating clinical stage Ⅱ,Ⅲ of cervical cancer patients were higher than those of the above two indexes alone (P<0.05).Conclusion:CK10 is abnormally low in the tissues of patients with cervical cancer,while Ki-67 is abnormally high in the tissues of patients with cervical cancer.Combined detection of its expression level may assist in the evaluation of clinical FIGO stage and lymph node metastasis in patients with cervical cancer.