硅烷化玻璃酸酯对皮肤屏障相关基因和蛋白的作用研究

Effects of silanol hyaluronic acid compound (SHAC) on skin barrier genes and proteins

主要通过qRT-PCR及免疫荧光(IF)法,研究了硅烷化玻璃酸酯(SHAC)对于皮肤屏障相关的8种基因表达和2种屏障相关蛋白合成的影响。选取合适样品浓度检测培养24 h后角质形成细胞中的FLG、LOR、IVL、KRT10、KRT16、TGM1、CLDN1、OCLN 8种基因表达量,同时检测角质形成细胞中ZO-1、CLDN1 2种蛋白的表达量。结果表明,SHAC在浓度0.625%时能显著促进IVL、TGM1、OCLN、CLDN1基因的表达,在浓度为2.5%时能显著促进OCLN、TGM1基因的表达;当SHAC浓度在0.625%时,能更好促进CLDN1基因的表达并能显著促进CLDN1蛋白含量上升,对ZO-1蛋白作用不明显,SHAC具有潜在的屏障修护作用。

The effects of silanol hyaluronic acid compound (SHAC) on the expression of 8 skin barrier related genes and the synthesis of 2 skin barrier related proteins were studied by qRT-PCR and immunofluorescence methods.The expression levels of FLG,LOR,IVL,KRT10,KRT16,TGM1,CLDN1 and OCLN genes in keratinocytes after 24 h culture were detected at appropriate sample concentration,and the expression levels of ZO-1 and CLDN1 proteins in keratinocytes were also detected.The results showed that the expression of IVL,TGM1,OCLN and CLDN1 could be significantly promoted at an SHAC concentration of 0.625%,while the expression of OCLN and TGM1 could be significantly promoted at an SHAC concentration of 2.5%.Especially when SHAC concentration was 0.625%,CLDN1 gene expression could be significantly promoted.When SHAC concentration was 0.625%,CLDN1 protein content was significantly increased,but no obvious effect was observed for ZO-1 protein.The results show that SHAC has potential barrier repair function.