高通量研磨-X射线荧光光谱法测定不同植源性食品中硒含量

Determination of Selenium Content in Different Plant Foods by High-throughput Lapping-X-ray Fluorescence Spectrometry

为了提高食品的样品检验效率,采用高通量研磨制备样品,利用X射线荧光光谱法对食品中硒含量进行检测。重点研究了样品含水率、样品颗粒粒径、研磨条件、检测条件等因素对检测结果的影响。结果表明:样品干基含水量小于20%,样品颗粒粒径小于100μm;高通量研磨条件为装样量3 g,频率30 Hz,研磨3 min;高精度X射线荧光光谱仪检测条件为检装样量0.2 g,PP膜压紧,检测90 s时的情况下,硒的检出限0.01 mg/kg,仪器的精密度小于5%,与粉碎法进行对比,相对偏差在-10.37%~8.05%。说明高通量研磨-X射线荧光光谱法检测食品中硒含量的方法快速、准确,可以满足批量样品现场检测分析需求。

In order to improve the inspection efficiency of plant samples,the samples were prepared by high-throughput grinding,and the selenium content in plants was detected by X-ray fluorescence spectrometry.The effects of moisture content,particle size,grinding conditions and testing conditions on the testing results were studied.The results showed that the dry basis water content and the particle size of the sample was less than 20%and 100μm,respectively.The high-throughput grinding conditions were as follows:sample loading 3 g,frequency 30 Hz,grinding 3 min.And high-precision X-ray fluorescence spectrometer detection conditions:the detection limit of selenium was 0.01 mg/kg for 90 s.The precision of the instrument was less than 5%.Compared with the comminution method,the relative deviation was between-10.37%and 8.05%.These results indicate that highthroughput grinding-X-ray fluorescence spectrometry is a fast and accurate method for the determination of selenium content in plants,which can meet the requirements of on-site detection and analysis of batch samples.