插穗选取部位对雌雄葎草生理基础及成活率的影响

Effects of Cuttings Selection Parts on the Physiological Basis and Survival Rate of Male and Female Humulus Scandens Seedlings

在雌雄异株攀缘植物葎草(Humulus scandens)成熟茎的不同部位,选取材料进行扦插,测定可溶性糖(SS)、蔗糖(SU)、淀粉(ST)、葡萄糖(GLU)等糖分含量,及丙酮酸(PA)含量和硝酸还原酶活性(NR),分析扦插成活率与生理代谢的性别差异,探究提高扦插成活率的方法。结果表明,雌株茎段的糖和氮代谢高于雄株(P<0.05),雄株茎段的呼吸作用高于雌株(P<0.05);插穗选取部位糖分含量主要受影响,性别次之;选取部位对雌株插穗的糖含量和NR活性影响大于雄株(P<0.05),而对PA含量影响小于雌株;雌株比雄株易于扦插繁殖,离根越近插穗成活率越高。

Humulus scandens as an dioecious climbing plant, because difficult to identify seedlings gender before flowering, so study the physiological basis of rooting and increase the survival rate of cuttings was required for studying the resistance difference of the male and female seedlings.Cuttings(from bottom to toplabeled as S,S,S) were selected from mature stems to determine soluble sugar(SS),sucrose(SU),starch(ST),glucose(GLU) content and pyruvate(PA) content and nitrate reductase activity(NR),then to analyzed gender differences of espiratory intensity and nitrogen metabolism rate.The results showed that: The sugar and nitrogen metabolism of female cuttings were higher than males(P<0.05),and the respiration of male cuttings was higher than females(P<0.05).The sugar content of the cuttings was mainly affected by the selected parts, followed by genders.The effect of selection parts on sugar content and NR activity of female cuttings was greater than males(P<0.05),while PA content was less than that of females.The survival rate of female cuttings was significantly higher than males, and the survival rate of Sparts was higher than Sand S.