基于三种多糖与酪蛋白复配制备W/O/W型乳液及其包封红景天苷效果的研究

Study on the Preparation of W/O/W Emulsion Based on Three Polysaccharides Mixed with Casein and Its Encapsulation Effect of Salidroside

为提高红景天苷稳定性及口服吸收效果,本研究以红景天苷为内水相,含聚甘油蓖麻醇酯的菜籽油为油相,分别采用葡聚糖、壳聚糖和大豆多糖混合酪蛋白作为外水相,制备W/O/W型多重乳液。研究了不同浓度的三种多糖对多重乳液的微观结构、粒径、电位、贮藏稳定性、乳化性、包埋率、载药量和体外消化过程的影响。结果显示,相比于酪蛋白对照组、壳聚糖-酪蛋白组和大豆多糖-酪蛋白组,葡聚糖-酪蛋白W/O/W型多重乳液稳定性最好。高浓度的葡聚糖能够明显提升葡聚糖-酪蛋白多重乳液的贮藏稳定性,当葡聚糖添加量为1.2%时,多重乳液平均粒径最小,可达623.03±5.21 nm(P<0.05);电位绝对值最高(P<0.05),平均电位为-37.3±0.46 mV;对酪蛋白乳化性质提升最大(P<0.05);对红景天苷的包埋率最高(P<0.05),可达92.8%,载药量为162.89±4.21μg/g。模拟消化研究发现葡聚糖-酪蛋白多重乳液不仅能有效地保护红景天苷,还能够靶向地在模拟肠道内传递和释放,葡聚糖添加量为1.2%的葡聚糖-酪蛋白负载体系的稳定性和控释效果最佳。该研究结果可为红景天苷在食品和医药中的应用提供理论支撑。

To improve the stability and oral absorption effect of salidroside, W/O/W multiple emulsion was prepared with salidroside as the internal aqueous phase and rapeseed oil containing polyglycerol polyricinoleate as the oil phase, and dextran, chitosan and soybean polysaccharide mixed casein as the external aqueous phase. The effects of three polysaccharides with different concentrations on the microstructure, particle size, potential, storage stability, surface hydrophobicity, emulsifying property, embedding rate, drug loading, and in vitro digestion of multiple emulsions were studied. The results showed that compared with the casein control group, chitosan-casein group, and soybean polysaccharide-casein group, dextran-casein W/O/W multiple emulsion had the best stability. A high concentration of dextran could significantly improve the storage stability of dextran-casein multiple emulsions. When the addition of dextran was 1.2%, the average particle size of the multiple emulsions was the smallest, up to 623.03±5.21 nm(P<0.05). The absolute value of potential was the highest(P<0.05), and the average potential was-37.3±0.46 mV. The emulsifying property of casein was improved the most(P<0.05). The embedding rate of salidroside was the highest(P<0.05), up to92.8%, and the drug loading was 162.89±4.21 μg/g. The study of simulated digestion showed that the dextran-casein multiple emulsion could not only effectively protect the salidroside, but also target the delivery and release in the simulated intestine. The dextran-casein loading system with the addition of 1.2% dextran had the best stability and controlled release effect. The research results can provide theoretical support for the application of salidroside in both food and medicine.