水培法鉴定甘薯抗甘薯瘟病技术的构建

Construction of identification of sweetpotato resistance to Ralstonia Solanacearum by hydroponics

为建立简便、快速、准确、可靠的甘薯抗甘薯瘟病的鉴定方法,研究建立了水体通气水培法和多孔定植块水培法2种鉴定技术,均能有效鉴定甘薯对甘薯瘟病的抗性.通过探索不同通气时间、接菌浓度对甘薯瘟病发生的影响,确定1×10^(7) CFU/mL菌体悬浮液和全时段通气为通气水培法进行甘薯抗甘薯瘟病鉴定的最佳条件.另外,采用1×10^(8) CFU/mL菌液接种后,使用透气保湿性佳的基质块进行定植,完成鉴定仅需7~10 d,比用土壤基质定植鉴定时间(14~21 d)缩短一半.建立的水培法丰富了甘薯抗甘薯瘟病鉴定的方法,有利于批量快速筛选抗甘薯瘟病的甘薯品种(系).

In order to fast, simply and accurately identify the resistance of sweetpotato to bacterial wilt, aerobic hydroponics and planting block substrate-applying hydroponics were established in this study. Both methods can effectively identify sweetpotato bacterial wilt resistance. By exploring the influence of different ventilation time and inoculation concentration for the accuracy of aeration hydroponics, it was determined that the inoculation bacteria concentration of 1×10^(7) CFU/mL and the full-time ventilation were the best conditions for aeration hydroponics. When the inoculation concentration was 1×10^(8) CFU/mL, using planting block substrate with good air permeability and water retention could not only ensure the identification effect, but also reduce the identification time to 7-10 d, which was almost half time compared to that of 14-21 d required for soil substrate colonization. The two hydroponics methods can optimize and enrich the indoor resistance identification methods to sweetpotato bacterial wilt. It is of great significance for quickly identifying a large amount of sweetpotato varieties’ disease resistance.