miR-199a-5p在宫颈癌患者癌组织及血清中的表达及临床意义

Expression and clinical significance of miR-199a-5p in cancer tissue and serum of patients with cervical cancer

目的 探讨miR-199a-5p在宫颈癌患者癌组织及血清中的表达及临床意义。方法 选取2016年6月至2018年10月河北省邯郸市中心医院收治的宫颈癌患者100例为宫颈癌组,另选取同期体检的健康女性100例为对照组。采用实时荧光定量聚合酶链反应(qPCR)分别检测宫颈癌患者癌组织、对应癌旁组织,以及两组血清中miR-199a-5p的相对表达水平;对比分析miR-199a-5p在不同样本中的表达及其与患者临床特征的关系;采用受试者工作特征(ROC)曲线分析miR-199a-5p诊断宫颈癌的临床价值;采用Kaplan-Meier生存曲线分析miR-199a-5p与宫颈癌患者3年总生存率的关系。结果 宫颈癌患者癌组织中miR-199a-5p的相对表达水平明显高于癌旁组织,宫颈癌组血清miR-199a-5p的相对表达水平明显高于对照组,差异均有统计学意义(P<0.05)。不同淋巴结转移情况和国际妇产科联盟(FIGO)分期的宫颈癌患者癌组织及血清中miR-199a-5p的相对表达水平差异有统计学意义(P<0.05);而在年龄、肿瘤最大径、绝经、病理分型、分化程度方面,宫颈癌患者癌组织及血清中miR-199a-5p的相对表达水平差异无统计学意义(P>0.05)。宫颈癌患者癌组织、血清中miR-199a-5p诊断宫颈癌的曲线下面积(AUC)分别为0.808(95%CI:0.651~0.961)、0.748(95%CI:0.537~0.960),诊断效能较高。生存分析结果表明,宫颈癌患者癌组织和血清miR-199a-5p低表达者3年总生存率均显著高于miR-199a-5p高表达者(P<0.05)。结论 miR-199a-5p在宫颈癌患者癌组织及血清中表达均明显上调,且其相对表达水平与宫颈癌淋巴结转移、FIGO分期以及患者生存率有关,miR-199a-5p可作为宫颈癌早期诊断及预后评估的又一指标。

Objective To investigate the expression and clinical significance of miR-199 a-5 p in cancer tissue and serum of patients with cervical cancer.Methods A total of 100 patients with cervical cancer admitted to Handan Municipal Central Hospital of Hebei Province from June 2016 to October 2018 were selected as the cervical cancer group, and other 100 healthy women undergoing physical examination in this hospital during the same period were selected as the control group.The real-time quantitative PCR(qPCR) was used to detect the expression levels of miR-199 a-5 p in cancer tissues, corresponding paracancerous tissues and serum in the two groups.The expression level of miR-119 a-5 p in different samples and its relationship with the clinical characteristics in the patients were comparatively analyzed.The receiver operating characteristic(ROC) curve was used to analyze the clinical value of miR-199 a-5 p in diagnosing cervical cancer;the Kaplan-Meier survival curve was used to analyze the relationship between the miR-199 a-5 p level and the 3-year overall survival rate in the patients with cervical cancer.Results The relative expression level of miR-199 a-5 p in cancer tissue of the patients with cervical cancer was significantly higher than that in paracancerous tissue, and the relative expression level of miR-199 a-5 p in serum of the cervical cancer group was significantly higher than that in control group, and the differences were statistically significant(P<0.05).In different lymph node metastasis and FIGO stages, the relative expression levels of miR-199 a-5 p in cancer tissue and serum of the patients with cervical cancer had statistical difference(P<0.05).The cancer tissue and serum relative expression levels of miR-199 a-5 p in the patients with cervical cancer had no statistical difference in the aspects of the age, maximal tumor diameter, menopause, pathological type and differentiation degree(P>0.05).The area under the ROC curve(AUC) of miR-199 a-5 p levels in cancer tissue and serum of the patients with cervical cancer for diagnosing cervical cancer were 0.808(95%CI:0.651-0.961) and 0.748(95%CI:0.537-0.960),respectively, indicating the diagnostic efficiency was higher.The survival analysis results showed that the 3-year overall survival rate of cervical cancer patients with low expression level of miR-199 a-5 p in cancer tissue and serum was significantly higher than that of the patients with high miR-199 a-5 p expression level(P<0.05).Conclusion The expression level of miR-199 a-5 p in cancer tissue and serum of the patients with cervical cancer is significantly up-regulated, moreover, its relative expression level is correlated with the cervical cancer lymph node metastasis, FIGO stage and patients′ survival rate.miR-199 a-5 p may serve another indicator of early diagnosis and prognostic evaluation of cervical cancer.