TIM-3及其配体在子宫腺肌病组织中的表达及意义

Expression and significance of TIM-3 and its ligand in adenomyosis tissues

目的 探讨T细胞免疫球蛋白及黏蛋白分子3(TIM-3)及其配体半乳凝集素9(Gal-9)在子宫腺肌病(UA)组织中的表达及意义。方法 选取2019年9月至2021年9月在该院因UA行子宫全切术的患者50例,术中剥离在位内膜组织(EU组)和异位内膜组织(EC组)。另选取因子宫颈上皮内瘤变Ⅲ级行子宫全切术的患者50例,术中取正常子宫内膜组织(对照组)。采用免疫印迹法检测组织中TIM-3、Gal-9、白细胞介素(IL)-6、IL-8蛋白表达水平;采用实时荧光定量PCR检测TIM-3、Gal-9、IL-6、IL-8基因表达水平。分析TIM-3、Gal-9蛋白表达水平与月经量和痛经的关系。结果 EU组和EC组TIM-3、Gal-9、IL-6、IL-8基因和蛋白表达水平均高于对照组,且EC组高于EU组(P<0.05)。EU组TIM-3蛋白表达水平分别与IL-6、IL-8蛋白呈正相关(r分别为0.297、0.325,P<0.05);Gal-9蛋白表达水平也分别与IL-6、IL-8蛋白呈正相关(r分别为0.384、0.302,P<0.05)。EC组TIM-3蛋白表达水平分别与IL-6、IL-8蛋白呈正相关(r分别为0.286、0.309,P<0.05);Gal-9蛋白表达水平也分别与IL-6、IL-8蛋白呈正相关(r分别为0.357、0.311,P<0.05)。与月经量正常患者比较,EU组和EC组中月经量过多患者TIM-3、Gal-9蛋白表达水平均较高(P<0.05)。EU组和EC组中痛经程度越高的患者TIM-3、Gal-9蛋白表达水平越高(P<0.05)。结论 TIM-3、Gal-9蛋白表达水平在UA病灶中明显上调,且与炎症因子IL-6、IL-8蛋白表达水平呈正相关,另外TIM-3、Gal-9蛋白还与患者月经量和痛经有关。

Objective To explore the expression and significance of T cell immunoglobulin and mucin molecule 3(TIM-3) and its ligand galectin-9(Gal-9) in adenomyosis(UA) tissues.Methods A total of 50 patients with hysterectomy due to UA in this hospital from September 2019 to September 2021 were selected, and the eutopic endometrial tissue(EU group) and ectopic endometrial tissue(EC group) were removed during the operation.In addition, 50 patients with total hysterectomy due to grade Ⅲ cervical intraepithelial neoplasia were selected, and the normal endometrial tissue was taken during the operation(control group).The Western blot was used to detect the protein expression levels of TIM-3,Gal-9,interleukin 6(IL-6) and IL-8 in the tissues;the real-time quantitative fluorescent PCR method was used to detect the gene expression levels of TIM-3,Ga1-9,IL-6 and IL-8.The relationship between TIM-3 and Gal-9 protein expression levels with the menstrual volume and algomenorrhea.Results The gene and protein expression levels of TIM-3,Gal-9,IL-6 and IL-8 in the EU group and EC group were higher than those in the control group, moreover the EC group was higher than the EU group(P<0.05).In the EU group, the TIM-3 protein expression level was positively correlated with IL-6 and IL-8 protein(r=0.297,0.325,P<0.05);the Gal-9 protein expression level was also positively correlated with IL-6 and IL-8 protein respectively(r=0.384,0.302,P<0.05).In the EC group, the TIM-3 protein expression level was positively correlated with IL-6 and IL-8 protein(r=0.286,0.309,P<0.05);the Gal-9 protein expression level was also positively correlated with IL-6 and IL-8 protein respectively(r=0.357,0.311,P<0.05).Compared with the patients with normal menstrual amount, the expression levels of TIM-3 and Gal-9 protein in patients with menorrhagia in the EU group and EC group were higher(P<0.05).The higher the degree of algomenorrhea, the higher the expression levels of TIM-3 and Gal-9 proteins in the EU group and EC group(P<0.05).Conclusion TIM-3 and Gal-9 protein expression levels are significantly up-regulated in UA lesions, moreover which are positively correlated with the expression levels of inflammatory factors IL-6 and IL-8 proteins.In addition, TIM-3 and Gal-9 proteins are also related to the menstrual volume and algomenorrhea.