漆酶的固定化技术及固定化漆酶载体材料研究进展

Research Progress of Laccase Immobilization Technology and Immobilized Laccase Carrier Materials

漆酶作为一种含铜的多酚氧化酶,可催化降解多种有机污染物且发生反应后唯一的产物是水。漆酶具有催化效率高、贮存要求低等优点,在酶催化领域得到了广泛的关注。漆酶固定化技术是通过物理或化学的方法将游离漆酶和相应载体材料结合起来。与游离漆酶相比,固定化漆酶表现出更高的重复使用性以及在温度、pH、储存、操作方面表现出更高的稳定性。结合目前固定化漆酶技术的研究现状和应用情况,文中介绍了漆酶的结构特征和催化特性,并综述了漆酶固定化技术和固定化漆酶载体材料的研究进展,指出了漆酶的固定化技术和载体材料目前存在的问题和未来的发展前景,旨在为进一步对固定化漆酶的研究和开发应用提供参考依据。

As a copper-containing polyphenol oxidase,laccase can make a role of catalytic degradation of many organic pollutants and the only product after the reaction is water.Due to its excellent catalytic efficiency,low storage requirements and other advantages,laccase has gained extensive attention in the field of enzyme catalysis.Laccase immobilization technology combines free laccase with corresponding carrier material by physical or chemical methods.Compared with free laccase,the immobilized laccase exhibits higher reusability and stability in temperature,pH,storage and operation.Combining the current research status and applications of the immobilized laccase technology,the structural and catalytic properties of laccase are introduced.The research progress of laccase immobilization technology and laccase immobilization carrier material is reviewed.It points out the current problems and future development prospects in laccase immobilization technology and carrier material.Furthermore,it aims to provide the reference to the research and development of laccase immobilization.