伊犁河谷罗布红麻资源调查及其黄酮抗氧化研究

Investigation on Apocynum venetum L.Resources in Ili River Valley and Anti-oxidative Activity of Their Flavonoids

罗布红麻具有重要的经济价值和生态应用潜力,但其资源分布、生物学特性等系统性研究的缺乏严重限制了新品种培育与产业开发进程。为探明伊犁河谷罗布红麻资源分布情况,评价种质间黄酮成分含量差异及其体外抗氧化活性,本研究对伊犁河谷地区6个市县罗布红麻开展了资源调查,并对其总黄酮抗氧化活性进行了分析。研究共在12个点位发现有罗布红麻资源分布,海拔分布在605~1376 m,株高介于75~220 cm之间,茎总黄酮含量变幅在15.92~42.31 mg RE/g DW,叶总黄酮含量变幅在34.33~56.06 mg RE/g DW。叶片中的总黄酮含量均高于茎,与海拔和株高无显著相关性(P<0.05)。对采集自察布查尔锡伯自治县罗布红麻资源样品的总黄酮抗氧化活性进行评价发现,其总黄酮对DPPH和ABTS自由基的清除率高达92.94%和95.15%,IC_(50)值分别为0.077 mg/mL和0.143 mg/mL,T-AOC总抗氧化能力达10.48μmol/mL。研究认为,新疆伊犁河谷罗布红麻资源黄酮含量变异丰富,具有较好的遗传改良潜力,并表现出优异的抗氧化特性,具有较高的开发利用价值。

Apocynum venetum L.is a crop species with important medicinal value and ecological application potential.However,its breeding and commercial marketing were strictly limited due to the lack of systematic research on its biological characteristics.In order to investigate the geographic distribution of Apocynum venetum L.resources in the Ili River Valley,and to evaluate the variations on flavonoid content among germplasms and their in vitro antioxidant activity,the germplasm resources in six districts and counties along the valley were surveyed,and their antioxidant activity of their total flavonoids were evaluated.Apocynum venetum L.resources were found at 12 sites,with an altitude of 605 to 1376 m.The germplasms showed 75 to 220 cm on plant height,with stem total flavonoid content of 15.92 to 42.31 mg RE/g DW,and leaf total flavonoid content of 34.33 to 56.06 mg RE/g DW.The total flavonoid content in leaves was higher than that in the stems,while total flavonoid content showed no correlation with either the collection site altitude or plant height(P<0.05).Especially,the samples collected from Apocynum venetum L.resources in Qapqal Xibe autonomous county showed that the total flavonoids scavenged 92.97%and 97.40%of DPPH and ABTS radicals with IC_(50) values of 0.077 mg/mL and 0.143 mg/mL,respectively,and the total antioxidant capacity of T-AOC reached 10.48μmol/mL.As a result,this study reported the rich genetic diversity on flavonoid content of Apocynum venetum L.resources in the Ili River Valley,suggesting a potential of their germplasm on genetic improvement and commercial marketing.