水稻DUF761基因家族成员鉴定与表达谱分析

Identification and Expression Profile Analysis of DUF761 Gene Family Members in Rice

DUF761家族是植物中一个未知功能结构域蛋白家族(DUF,domains of unknown function),其在水稻中的功能从未被探索。本研究通过生物信息学分析在水稻全基因组共鉴定到33个水稻DUF761基因家族成员。系统发育分析将这些成员分为3个亚家族。保守基序Motif-1分布于所有家族成员。在OsDUF761基因启动子区域共发现15种与胁迫响应、激素响应和发育相关的顺式作用元件。有30个基因的启动子区域含有ABRE元件(脱落酸响应),其中OsDUF761-21的启动子含有16个ABRE元件。组织表达谱检测结果表明,不同的OsDUF761基因成员在水稻中具有相似或者迥异的组织表达谱。在ABA、JA、低温、干旱和稻瘟菌分别处理水稻后,水稻中分别有12、13、24、24和18个OsDUF761基因的转录表达水平发生显著变化;其中,有8个基因在ABA处理和干旱处理后表现出共同的显著上调或者下调响应趋势;有6个基因在JA处理和稻瘟菌处理后表现出共同的响应趋势。本研究对水稻DUF761基因家族进行了全面分析,有助于进一步明确DUF761家族成员的生物学功能。

The DUF761 family members contain a domain of unknown function(DUF)protein in plants,and these functions in rice remain un-described.In this study,33 family members in rice genome have been identified by bioinformatics pipeline.Phylogenetic analysis suggested three subfamilies,while Motif-1,a conserved motif was present in each member of each subfamily.Fifteen types of cis-acting elements related to stress response,hormone response and development were found in the promoters of these OsDUF761 genes.The promoters of 30 OsDUF761 genes contain the ABRE element(abscisic acid response),and the promoter of a member OsDUF761-21 contains 16 ABRE elements.Tissue expression profile analysis showed that OsDUF761 gene members represented similar or distinct tissue expression profile patterns in rice.The transcriptional expression levels of 12,13,24,24 and 18 OsDUF761 genes in rice were significantly changed after ABA,JA,low temperature,drought and rice blast treatments,respectively.Eight genes showed a common significant upor down-regulation response trend after ABA treatment and drought treatment.Six genes showed a common response trend after JA treatment and M.oryzae treatment.Collectively,by conducting a comprehensive analysis of the rice DUF761 gene family members,this study provided insights for future deciphering their biological functions in rice.