东风螺、弯竹蛏和斑节对虾的壳基质蛋白组学分析

Shell Matrix Proteins Analysis of Babylonia areolates,Solen arcuatus and Penaeus monodon

从软体动物中的腹足纲、双壳纲以及节肢动物中的甲壳纲分别选取了3种中国沿海常见的品种——东风螺、弯竹蛏、斑节对虾,提取并分析它们的壳基质蛋白。利用液相色谱串联质谱(LC-MS/MS)技术对蛋白质组学进行分析发现特定的蛋白质具有明显的种属差异:①N66蛋白(一种碳酸酐酶)通常存在于弯竹蛏中,但不存在于东风螺和斑节对虾中,揭示N66蛋白质具有特异性,在未来的物种鉴定中可能具有重要的参考意义;②角质层蛋白AM1199和CP14.6存在于斑节对虾的表皮结构中,在东风螺和弯竹蛏中未检测到;③血蓝蛋白同时在3种动物外壳的基质蛋白中被鉴定出来,揭示了其在不同物种的外壳形成与发育过程中的重要作用。该研究为壳基质蛋白在软体动物或节肢动物外壳中的鉴定提供了基础,也为探索不同物种的生物矿化过程提供了重要参考。

For the study,three species commonly occurring in the coastal waters of China,i.e.Babylonia areolates,Solen arcuatus and Penaeus monodon,are respectively selected from the Gastropoda and Bivalvia of mollusks and the Crustacea of arthropods and their shell matrix proteins are extracted and analyzed.The proteomics analysis by using liquid chromatography tandem mass spectrometry(LC-MS/MS)reveals that the given protein has significant species differences:①rotein N66(a kind of carbonic anhydrase)occurs commonly in Solen arcuatus,but not in Babylonia areolates and Penaeus monodon.revealing that the protein N66 has the specificity,which may have important reference significance in species identification in the future;②uticle proteins AM1199 and CP14.6 are generally found in the epidermal structure of Penaeus monodon,and not detected in Babylonia areolates and Solen arcuatus;③emocyanin can be identified in the shell matrix proteins of all the three animals,revealing its important role in the shell formation and growth of different species.This study can provide a foundation for the identification of shell matrix proteins in mollusk or arthropod shells and also an important reference for exploring the biomineralization process in different species.