发酵性丝孢酵母处理精炼大豆油废水的工艺优化及动力学分析

Technical Study and Kinetic Analysis of the Treatment of Refined Soybean Oil Wastewater by Trichosporon fermentans

在5 L搅拌式反应器内利用发酵性丝孢酵母处理精炼大豆油废水,并采用正交试验优化处理条件,通过极差分析得到精炼大豆油废水生物处理的较佳条件:温度28℃、转速300 r/min、进气量2 L/min和接种量10%。在该条件下运行36 h后精炼大豆油废水的化学需氧量和含油量的去除率分别为97.31%和89.09%,生物量和油脂分别达到9.27 g/L和51.9%。通过Monod、Tessier和Moser模型研究了发酵性丝孢酵母生物量增长和精炼大豆油废水的污染物降解,发现相比于Monod和Moser模型,Tessier模型更适合研究微生物的增长,并建立了发酵性丝孢酵母生物量增长的动力学方程式,所得到的动力学参数可用于评估含油废水生物处理反应器的设计和运行。

The treatment of the refined soybean oil wastewater by Trichosporon fermentans was optimized by orthogonal test in a 5 L stirred reactor.By the range analysis,the optimal conditions for biological treatment of refined soybean oil wastewater were temperature of 28℃,rotating speed of 300 r/min,air intake of 2 L/min,and inoculation amount of 10%.After 36 h operation under these conditions,the removals of COD and oil content of the refined soybean oil wastewater were 97.31%and 89.09%,and the biomass and lipid content reached 9.27 g/L and 51.9%,respectively.The Monod,Tessier,and Moser models were applied to study the biomass growth of Trichosporon fermentans and the pollutant degradation of the refined soybean oil wastewater,and it was found that the Tessier model was more suitable for studying the growth of microorganisms than those of the Monod and Moser models.The kinetic equations for the biomass growth of Trichosporon fermentans were established,and the corresponding parameters were obtained and applied to assess the design and operation of a bioreactor for the treatment of oily wastewater.