三相分离法制备金针菇提取物中多糖的理化性质及体外活性

Physicochemical Properties and in vitro Activities of Polysaccharides from Flammulina filiformis Extract Prepared by Three-Phase Partitioning

采用三相分离法制备金针菇(Flammulina filiformis)提取物,以乙醇沉淀法为对照,考察提取物得率、蛋白含量、多糖含量、单糖组成、多糖重均分子量分布及体外抗氧化和免疫活性。结果表明:与乙醇沉淀法制备的提取物(FVP 70)相比,三相分离法制备的提取物(TTPFVP)得率显著降低,但其中多糖含量极显著增加;TTPFVP由摩尔比为2.6∶10.8∶13.3∶1.0∶4.7∶0.1的岩藻糖、半乳糖、葡萄糖、木糖、甘露糖和葡萄糖醛酸组成,主要含有重均分子量为2.36×10^(7)和4.77×10^(5)的两个多糖组分,FVP 70由摩尔比为6.2∶1.0∶23.3∶25.7∶4.2∶11.3∶0.3的岩藻糖、葡萄糖胺、半乳糖、葡萄糖、木糖、甘露糖和葡萄糖醛酸组成,主要含有一个重均分子量为3.31×10^(5)的多糖组分;TTPFVP的DPPH自由基清除能力、ABTS自由基清除能力、Fe离子还原能力及刺激RAW264.7释放NO的作用明显优于FVP70。

The three-phase partitioning(TPP) method was used to extract crude polysaccharides from Flammulina filiformis. Using the extract obtained by the traditional alcohol precipitation method(FVP70) as the control, the TPP derived crude polysaccharide extract(FVPTPP) was measured for extraction yield, protein content, polysaccharide content, monosaccharide composition, weight-average molecular weight distribution, and in vitro antioxidant and immunological activities. The results showed that the extraction yield of FVPTPP( 2. 30 %) was lower than that of FVP 70. However, FVPTPP had a significantly higher polysaccharide content of 40. 53 %. TTPFVP consisted of fucose, galactose, glucose, xylose, mannose and glucuronic acid in the molar ratio of 2. 6∶10. 8∶13. 3∶1. 0∶4. 7∶0. 1, and mainly contained two polysaccharide components with a weight average molecular weight of 2. 36 × 10^(7) and 4. 77 × 10^(5), respectively. On the other hand, FVP70consisted of fucose, glucosamine, galactose, glucose, xylose, mannose and glucuronic acid in the molar ratio of 6. 2∶1. 0∶23. 3∶25. 7∶4. 2∶11. 3∶0. 3, and mainly contained one polysaccharide component with a weight average molecular weight of 3. 31 × 10^(5). Compared with FVP 70, TPPFVP showed stronger DPPH and ABTS scavenging ability, ferric reducing ability, and stimulation of NO release from RAW 264. 7 cells.