HE染色在临床病理诊断中的应用价值研究

Study on application value of HE staining in clinicopathological diagnosis

目的探究苏木精-伊红染色法(HE染色)在临床病理诊断中的应用价值。方法72张病理组织石蜡切片为研究对象,随机分为对照组及实验组,每组36张。对照组实施常规HE染色,实验组实施改进HE染色。比较两组病理诊断符合率。结果实验组骨制片、子宫内膜制片、脂肪组织制片、胃肠肝胆制片诊断符合率分别为85.71%、87.50%、77.78%、91.67%,对照组骨制片、子宫内膜制片、脂肪组织制片、胃肠肝胆制片诊断符合率分别为50.00%、55.56%、50.00%、63.64%。实验组病理诊断总符合率为86.11%,高于对照组的55.56%,差异有统计学意义(P<0.05)。结论病理诊断中实施改进HE染色可更好提高诊断的符合率,以此为疾病的诊治提供参考依据,意义重大。

Objective To investigate the application value of hematoxylin-eosin(HE)staining in clinicopathological diagnosis.Methods A total of 72 paraffin sections of pathological tissues were selected as the research subjects,and were randomly divided into the control group and the experimental group,with 36 pieces in each group.The control group was subjected to conventional HE staining,and the experimental group was subjected to improved HE staining.The coincidence rate of pathological diagnosis between the two groups was compared.Results In the experimental group,the diagnostic coincidence rates of bone slices,endometrial slices,adipose tissue slices,and gastrointestinal hepatobiliary slices were 85.71%,87.50%,77.78%,and 91.67%;in the control group,the diagnostic coincidence rates of bone slices,endometrial slices,adipose tissue slices,and gastrointestinal hepatobiliary slices were 50.00%,55.56%,50.00%,and 63.64%.The coincidence rate of pathological diagnosis in the experimental group was 86.11%,which was higher than 55.56%in the control group,and the difference was statistically significant(P<0.05).Conclusion Improved HE staining in pathological diagnosis can better improve the diagnostic coincidence rate,and provide a reference for the diagnosis and treatment of diseases,which is of great significance.