B细胞抑制性受体FcγRIIB与CD45分子相互关系的研究

A study on the relationship between inhibitory receptor FcγRIIB and CD45 molecule of B cell

目的 研究B细胞抑制性受体FcγRIIB与CD45分子之间的相互关系。方法 设计FcγRIIB-shRNA连接到反转录病毒载体上,通过反转录病毒将其导入A20细胞检测FcγRIIB-shRNA的敲低效率。然后将FcγRIIB-shRNA通过反转录病毒导入CD45.1小鼠脾脏B细胞,利用流式细胞术检测B细胞表面CD45.1分子的表达。通过流式细胞术检测FcγRIIB敲除小鼠的脾脏B细胞表面CD45.2分子的表达,研究B细胞表面抑制性受体FcγRIIB与CD45分子之间的关系。结果 与对照组相比,通过shRNA敲低FcγRIIB的表达会导致小鼠B细胞表面CD45.1分子的表达水平显著降低,并且FcγRIIB敲除小鼠B细胞表面CD45.2分子的表达也显著低于野生小鼠。结论 B细胞的抑制性Fc受体FcγRIIB影响其表面CD45分子的表达,表明FcγRIIB与CD45分子之间存在免疫调节网络,对于深入研究自身免疫性疾病的发生发展有着重要的意义。

Objective To study the relationship between inhibitory receptor FcγRIIB and CD45 molecule of B cell.Methods A FcγRIIB-shRNA vector was designed and transduced to A20 cells by retroviruses to test the knockdown efficiency.Then,Fcγ RIIB-shRNA was transduced into CD45.1 mouse spleen B cells via retrovirus,and the expression of CD45.1was detected by flow cytometry.In addition,the expression of CD45.2 on the surface of spleen B cells of FcγRIIB knockout mice was detected by flow cytometry to investigate the relationship between the inhibitory receptor FcγRIIB and CD45.Results Knockdown of FcγRIIB by shRNA resulted in significantly lower expression of CD45.1 on the B cell surface of mice compared with the control group,and the expression of CD45.2 on the B cell surface of FcγRIIB knockout mice was also significantly lower than that of wild mice.Conclusion The inhibitory Fc receptor FcγRIIB affects the expression of CD45 molecule on the surface of B cells,suggesting that there is an immune regulatory network between FcγRIIB and CD45 molecule,which is of great significance for further study on the occurrence and development of autoimmune diseases.