PI3K/Akt信号通路相关蛋白在胃癌细胞中的表达及其意义

Expression and significance of PI3K/Akt signaling pathway related proteins in gastric cancer cells

目的探讨磷脂酰肌醇3激酶(phosphoinositide 3-kinase,PI3K)/蛋白激酶B(protein kinase B,PKB/Akt)信号通路中相关蛋白磷酸化PI3K(phosphorylated phosphoinositide 3-kinase,p-PI3K)和磷酸化蛋白激酶B(phosphorylated protein kinase B,p-Akt)在胃癌组织中的表达与胃癌相关病理参数之间的关系。方法收集2017年1月至2018年12月南昌大学第二附属医院经胃镜病理确诊为胃癌并行手术切除患者的胃癌标本(n=100)和对应癌旁组织(n=100),以及正常胃黏膜组织(n=48),比较3种组织中p-PI3K和p-Akt的表达情况,探讨PI3K/Akt信号通路相关蛋白与胃癌相关病理参数之间的关系。结果p-PI3K在胃癌组织、癌旁组织及正常胃黏膜中的阳性表达率分别为55.0%、23.0%及14.6%;p-Akt在胃癌组织、癌旁组织及正常胃黏膜中的阳性表达率分别为29.0%、13.0%及8.3%,胃癌组织中p-PI3K、p-Akt的表达率均显著高于癌旁组织及正常胃黏膜,差异均有统计学意义(P<0.05)。胃癌组织中p-PI3K、p-Akt的表达与患者的年龄、性别无明显相关性,差异无统计学意义(P>0.05)。肿瘤病理分期为Ⅲ~Ⅳ期、低分化、浸润深度穿透浆膜及淋巴结转移的患者胃癌组织中p-PI3K、p-Akt的阳性率更高(P<0.05)。结论p-PI3K和p-Akt在胃癌组织中高表达,且与胃癌的相关病理参数存在显著相关性,PI3K/Akt信号通路可能参与了胃癌的发生与发展。

Objective To investigate the relationship between the expression of related proteins phosphorylated phosphoinositide 3–kinase(p–PI3K) and phosphorylated protein kinase B(p–Akt) in PI3K/Akt signal transduction pathway in gastric cancer cells and related pathological parameters of gastric cancer. Methods The gastric cancer specimens(100 cases) and corresponding tumor–adjacent tissues(100 cases), as well as normal gastric mucosa tissues(48cases) were collected from January 2017 to December 2018 in the Second Affiliated Hospital of Nanchang University who were diagnosed with gastric cancer by gastroscopy and underwent surgical resection. The expression of p–PI3K and p–Akt in the three tissues were compared and explored the relationship between PI3K/Akt signaling pathway related proteins and gastric cancer related pathological parameters. Results The positive expression rates of p–PI3K in gastric cancer tissues, tumor–adjacent tissues and normal gastric mucosa were 55.0%, 23.0% and 14.6%, respectively. The positive expression rates of p–Akt in gastric cancer tissues, tumor–adjacent tissues and normal gastric mucosa were 29.0%,13.0% and 8.3%, respectively. The expression rates of p–PI3K and p–Akt in gastric cancer tissues were significantly higher than those in tumor–adjacent tissues and normal gastric mucosa, the difference were statistically significant(P<0.05). There was no significant correlation between the expression of p–PI3K and p–Akt in gastric cancer tissues and the age and gender of patients, the differences were not statistically significant(P>0.05). The positive rates of p–PI3K and p–Akt in gastric cancer tissues were higher in patients with pathological stages Ⅲ–Ⅳ, low differentiation, deep infiltration through serous membrane and lymph node metastasis(P<0.05). Conclusion p–PI3k and p–Akt are highly expressed in gastric cancer tissues and significantly correlated with related pathological parameters of gastric cancer, indicating that the PI3K/Akt signaling pathway may be involved in the occurrence and development of gastric cancer.