慢性阻塞性肺疾病患者外周血核苷酸结合寡聚化结构域样受体蛋白3水平与肺功能、病情程度和预后的关系

Relationship between peripheral blood nucleotide binding oligomerization domain like receptor protein 3 level and lung function,disease severity and prognosis of patients with chronic obstructive pulmonary disease

目的分析慢性阻塞性肺疾病(COPD)患者外周血核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)水平与肺功能、病情程度和预后的关系。方法选择邯郸市中心医院2018年1月至2019年6月收治的COPD患者102例为研究对象(观察组),另选择同期来院体检健康者40例作为对照组。根据预后情况将观察组患者又分为死亡组(n=37)和存活组(n=65)。观察组患者于入院第2天、对照组受试者于体检当日采用肺功能仪测定第一秒用力呼气容积(FEV_(1))占预计值(Pred)的百分比(FEV_(1)%/Pred)、FEV_(1)占用力肺活量(FVC)的百分比(FEV_(1)/FVC)。观察组患者于入院第2天清晨、对照组受试者于体检当日抽取空腹外周静脉血约5 mL,采用酶联免疫吸附试验法测定血清中NLRP3水平。结果观察组患者血清中NLRP3水平显著高于对照组,FEV_(1)%/Pred和FEV_(1)/FVC显著低于对照组(P<0.05)。随着COPD患者病情严重程度分级的增加,患者血清中NLRP3水平逐渐升高,FEV_(1)%/Pred、FEV_(1)/FVC逐渐降低,两两比较差异均有统计学意义(P<0.05)。Pearson相关性分析结果显示,患者血清中NLRP3水平与FEV_(1)%/Pred和FEV_(1)/FVC均呈负相关(r=-0.661、-0.618,P<0.05)。死亡组患者血清中NLRP3水平显著高于存活组(P<0.05)。结论COPD患者血清中NLRP3水平高于健康人群,且随着病情的加重其水平呈上升趋势。NLRPS水平与COPD患者肺功能呈负相关关系。

Objective To analyze the relationship between the level of peripheral blood nucleotide binding oligomerization domain like receptor protein 3(NLRP3)and lung function,disease severity and prognosis of patients with chronic obstructive pulmonary disease(COPD).Methods A total of 102 COPD patients admitted to the Handan Central Hospital from January 2018 to June 2019 were selected as the study objects(observation group),and 40 healthy people who came to the hospital for physical examination during the same period were selected as the control group.The patients in the observation group were divided into death group(n=37)and survival group(n=65)according to the prognosis.On the second day of admission of patients in the observation group and on the day of physical examination of subjects in the control group,the ratio of forced expiratory volume in one second(FEV_(1))to predicted value(Pred)(FEV_(1)%/Pred)and the ratio of FEV_(1) to forced vital capacity(FEV_(1)/FVC)were measured by pulmonary function meter.On the morning of the second day of admission of patients in the observation group and on the day of physical examination of subjects in the control group,about 5 mL of fasting peripheral venous blood was taken and the serum NLRP3 level was determined by enzyme-linked immunosorbent assay.Results The serum NLRP3 level of patients in the observation group was significantly higher than that in the control group,while the FEV_(1)%/Pred and FEV_(1)/FVC of patients in the observation group was significantly lower than that in the control group(P<0.05).With the increase of COPD grade,the serum NLRP3 level of patients gradually increased,and the FEV_(1)%/Pred,FEV_(1)/FVC gradually decreased;the difference among patients with different grade was statistically significant(P<0.05).Pearson correlation analysis showed that the level of serum NLRP3 of patients was negatively correlated with FEV_(1)%/Pred and FEV_(1)/FVC(r=-0.661,-0.618;P<0.05).The level of serum NLRP3 of patients in the death group was significantly higher than that in the survival group(P<0.05).Conclusion The serum NLRP3 level of COPD patients is higher than that of healthy people,and the level of NLRP3 increases with the aggravation of the disease severity.The serum NLRP3 level is negatively correlated with lung function of COPD patients.