可移植人髓母细胞瘤PUMC-MBT1瘤株的建立与鉴定

Establishment and identification of a medulloblastoma transplantable tumor strain PUMC-MBT1

目的建立细胞系来源的异种种植肿瘤(CDX)体内可连续稳定传代的人髓母细胞瘤研究模型,并分析其生长和形态特点、病理特征。方法采用自建人髓母细胞瘤细胞系(PUMC-MB1)移植于SCID小鼠右侧腋窝,接种细胞数为5×10^(6)个/只。当细胞系来源的异种种植肿瘤(CDX)长径增长至1.5 cm时处死小鼠,收集肿瘤组织并进行体内传代操作,重复传代10次,于第10代时观察肿瘤生长特点及荷瘤小鼠的生存时间,收集肿瘤进行病理分析。结果CDX来源的人髓母细胞瘤瘤株已在体内连续传代至10代(命名为PUMC-MBT1),成瘤率为100%,在接种第14天左右可触及皮下结节,第29天移植瘤长径达到1.5 cm左右,荷瘤小鼠平均寿命50 d。病理学检查结果显示移植瘤为经典型髓母细胞瘤,Ki-67阳性(80%~90%),Syn阳性(40%~50%),NeuN阳性(20%~30%)。结论本课题组成功建立了人髓母细胞瘤移植瘤瘤株PUMC-MBT1,造模简单便捷,成瘤率高,病理学性质稳定,保持了经典型髓母细胞瘤的特性,为髓母细胞瘤研究提供了更多的体内研究模型。

Objective To establish a transplantable tumor strain of medulloblastoma in vivo from cell-line-derived xenograft(CDX)and to analyze the growth and pathological characteristics of the transplanted tumor model.Methods A previously established medulloblastoma cell line(PUMC-MB1)was utilized to construct CDX.The cultured cell suspension was injected into the right axilla of SCID mice subcutaneously with dosage as 5×10^(6) cells/mouse.The mice were sacrificed when the diameter of CDX reached around 1.5 cm.The xenografts were harvested and transplanted to other mice with the same number of cells.The process was repeated 10 times.The growth characteristics of transplanted medulloblastoma and survival curve of tumor-bearing mice at passage 10 were observed.The pathological characteristics of transplanted tumor were analyzed.Results The transplantable strain of medullo-blastoma with stable in vivo properties was named PUMC-MBT1.The successful rate of tumor transplantation was 100%at passage 10.The subcutaneous mass could be palpated 14 days after injection.The diameters of subcutaneous xenograft could reach about 1.5 cm 29 days after injection.Tumor-bearing mice died 50 days after injection.HE sections demonstrated that the transplanted PUMC-MBT1 belonged to classic medulloblastoma and the positive rate of Ki-67,Syn and NeuN were 80%-90%,40%-50%and 20%-30%respectively.Conclusions A successful animal model of medulloblastoma is established with easy manipulation,characteristic features of classic medulloblastoma,so it is a potential technology for in vivo research.