FABP4表达上调促进人子宫内膜癌细胞系HEC-1-A增殖和迁移

Up-expression of FABP4 promotes the proliferation and migration of endometrial cancer cell line HEC-1-A

目的探讨脂肪酸结合蛋白4(FABP4)在子宫内膜癌中的表达及其对子宫内膜癌细胞系HEC-1-A增殖、迁移及侵袭的影响。方法RT-qPCR检测子宫内膜癌组织及癌旁组织FABP4 mRNA的表达水平,分析FABP4表达与子宫内膜癌临床病理特征的相关性。将HEC-1-A细胞分为对照组、FABP4阴性对照(si-NC)组和干扰FABP4(si-FABP4)组。RT-qPCR检测HEC-1-A细胞FABP4 mRNA的表达;MTT法检测HEC-1-A细胞增殖;Transwell小室法检测细胞迁移与侵袭;免疫印迹法检测HEC-1-A细胞FABP4、基质金属蛋白酶(MMP)-2和MMP-9蛋白的表达。结果子宫内膜癌组织中FABP4 mRNA表达水平显著高于癌旁组织(P<0.05);FABP4表达与肿瘤TNM分期和淋巴结转移有关(P<0.05);干扰FABP4表达可显著抑制子宫内膜癌细胞增殖、迁移与侵袭能力。结论FABP4在子宫内膜癌中表达上调,干扰FABP4表达可抑制子宫内膜癌细胞增殖、迁移和侵袭。

Objective To investigate the expression of fatty acid binding protein 4(FABP4)in endometrial cancer and its effects on the proliferation,migration and invasion of endometrial cancer cells line HEC-1-A.Methods The expression level of FABP4 mRNA in endometrial cancer tissues and adjacent normal tissues was detected with RT-qPCR.The correlation between FABP4 mRNA expression and pathological changes of endometrial cancer was analyzed.HEC-1-A cells were cultured in vitro and were randomly divided into control group,FABP4 negative control(si-NC)group and interference FABP4(si-FABP4)group.RT-qPCR,MTT and Transwell assays were used respectively to detect the FABP4 mRNA expression in HEC-1-A cells,the proliferation,migration and invasion of HEC-1-A cells.Western blot was used to detect the protein expression of FABP4,matrix metalloproteinase(MMP)-2 and MMP-9 of HEC-1-A cells in each group.Results The expression level of FABP4 mRNA in endometrial cancer tissues was significantly higher than that in tissues adjacent to cancer(P<0.05);The FABP4 mRNA expression was related to tumor TNM staging and lymph node metastasis(P<0.05).Interference with FABP4 expression could significantly inhibit the proliferation,migration and invasion of endometrial cancer cells.Conclusions Expression of FABP4 is up-regulated in endometrial cancer and interference with FABP4 expression can inhibit the proliferation,migration and invasion of endometrial cancer cells.