淫羊藿苷改善重症急性胰腺炎模型大鼠肺损伤

Icariin ameliorates lung injury in severe acute pancreatitis of rat models

目的探讨淫羊藿苷(ICA)通过抑制炎性反应减轻重症急性胰腺炎(SAP)模型大鼠肺损伤及机制。方法将大鼠随机分为假手术组(sham组)、模型组(SAP组,将5%牛黄胆酸钠按0.1 mL/kg通过微泵逆行泵入胆管)和实验组(ICA组,造模前2 h给予淫羊藿苷80 mg/kg干预)。每组6只大鼠,造模24 h后收集下腔静脉血、胰腺组织和肺组织。HE染色观察胰腺和肺脏病理损伤;ELISA测定血清淀粉酶活性、IL-6、IL-1β和TNF-α含量;免疫组织化学染色测定肺组织中炎性因子IL-6、IL-1β和TNF-α含量;Western blot测定肺组织中磷酸化JNK/JNK和磷酸化NF-κB/NF-κB比值。结果SAP组胰腺和肺脏的病理评分显著高于sham组(P<0.01),ICA组胰腺和肺脏的病理评分较SAP组明显降低(P<0.01);SAP组血清淀粉酶活性、血清IL-6、IL-1β和TNF-α含量以及肺组织中IL-6、IL-1β和TNF-α表达量较sham组明显升高(P<0.01),而ICA组血清淀粉酶活性、血清IL-6、IL-1β和TNF-α含量以及肺组织中IL-6、IL-1β和TNF-α表达量较SAP组明显降低(P<0.01);SAP组肺组织磷酸化JNK和磷酸化NF-κB水平较sham组显著升高(P<0.01),而ICA治疗可以显著降低肺组织中磷酸化JNK和磷酸化NF-κB水平(P<0.01)。结论ICA可能通过抑制JNK/NF-κB信号通路介导的急性炎性反应,从而改善SAP模型大鼠肺损伤。

Objective To evaluate the protective effect and inflammation inhibition mechanism of icariin(ICA)on lung injury in rat model with severe acute pancreatitis(SAP).Methods The rats were randomly divided into sham operation group(sham group),model group(SAP group,5%sodium taurocholate was retrograde pumped into bile duct according to 0.1 mL/kg through micropump)and experimental group(ICA group,icariin 80 mg/kg intervention was given 2 hours before modeling).There were 6 rats in each group.Twenty-four hours after the establishment of the model,the inferior vena cava blood,pancreatic tissue and lung tissue were collected.The pathological injury of pancreas and lung was observed by HE staining;The activity of amylase,serum IL-6,IL-1βand TNF-αwere measured by ELISA;The expression of inflammatory factors IL-6,IL-1βand TNF-αin lung tissue was determined by immunohistochemical staining;And the ratio of phosphorylated JNK/JNK to phosphorylated NF-κB/NF-κB in lung tissue was measured by Western blot.Results The pathological scores of pancreas and lung in SAP group were significantly higher than those in sham group;And the pathological scores of pancreas and lung in ICA group were significantly lower than those in SAP group.Serum amylase activity,serum IL-6,IL-1βand TNF-αcontent and the expression of IL-6,IL-1βand TNF-αin lung tissue in SAP group were significantly higher than those in sham group;While serum amylase activity,serum IL-6,IL-1βand TNF-αcontent and the expression of IL-6,IL-1βand TNF-αin lung tissue in ICA group were significantly lower than those in SAP group.The level of phosphorylated JNK and phosphorylated NF-κB in lung tissue of SAP group were significantly higher than those of sham group;While icariin might significantly reduce the level of phosphorylated JNK and phosphorylated NF-κB in lung tissue.Conclusions ICA ameliorates lung injury in SAP rat model by inhibiting acute inflammation response,which is potentially mediated by JNK/NF-κB signal pathway.