墨旱莲不同药用部位UPLC特征图谱及5种成分含量测定研究

Study on UPLC Characteristic Chromatogram and Content Determination of Five Components of Different Medicinal Parts of Ecliptaprostrata L.

目的建立墨旱莲超高效液相色谱(UPLC)特征图谱及咖啡酸、木犀草苷、异绿原酸B、4,5-O-二咖啡酰基奎宁酸、蟛蜞菊内酯的含量测定方法,对墨旱莲不同药用部位的质量进行评价。方法采用Agilent SBC_(18)色谱柱(100 mm×2.1 mm,1.8μm),以乙腈-0.53%磷酸溶液(含0.8%三乙胺)为流动相进行梯度洗脱,流速0.3 mL/min,检测波长330 nm。采用外标法测定11批墨旱莲药材及其花、叶、茎中5种成分的含量。采用SPSS20.0软件对数据进行聚类分析和主成分分析。结果建立了墨旱莲UPLC特征图谱共有模式,确定了9个共有峰,指认了5个色谱峰,并测定不同药用部位5种成分的含量。通过聚类分析和主成分分析可将11批样品分为三类,与特征图谱和含量测定结果基本相符,表明墨旱莲不同药用部位的质量存在差异。结论本研究建立的方法简便,结果稳定、准确,可为墨旱莲药材的质量控制提供依据。

Objective To establish a UPLC method for the determination of caffeic acid,luteolin-7-O-glucoside and isochlorogenic acid B,4,5-O-dicaffeoylquinic acid,and wedelolactone of Ecliptaprostrata L.;To evaluate the quality of the different medicinal parts of Ecliptaprostrata L..Methods The UPLC analysis was performed on an Agilent SB C_(18) column(100 mm×2.1 mm,1.8μm),with a mobile phase consisting of acetonitrile 0.53%phosphate acid(containing 0.8%triethylamine)in gradient mode at a flow rate of 0.3 mL/min.The detection wavelength was 330 nm.The contents of the 5 index constituents in 11 batches of Ecliptaprostrata L.and its flower,leaf,stem were determined by external standard method.SPSS 20.0 was used for cluster analysis and principal component analysis.Results Common pattern of UPLC characteristic chromatogram of Ecliptaprostrata L.was established that 9 common peaks were confirmed and 5 of them were identified.The contents of these five index components in different medicinal parts were determined.The samples were divided into three categories by cluster analysis and principal component analysis,which were basically consistent with the characteristic chromatogram and content determination results,indicating that there were differences in the quality of different medicinal parts of Ecliptaprostrata L..Conclusions The proposed method is simple,stable and accurate,which can provide reference for the quality control of Ecliptaprostrata L..