不同蛋白含量大豆籽粒的转录组分析及蛋白合成相关基因预测

Transcriptome profiling and prediction of genes related to protein synthesis in soybean seeds with different protein contents

【目的】筛选大豆蛋白合成相关转录因子,预测蛋白合成候选基因和代谢通路。【方法】以低蛋白大豆材料中豆4901和高蛋白大豆材料淮豆98-28为研究对象,采用RNA-Seq技术,对不同蛋白含量的大豆籽粒进行高通量测序。【结果】获取到43.14 Gb Clean Data,每个样品数据量都在6.46 Gb以上,GC含量在45.73%以上,Q20碱基百分比在94.78%以上,Q30碱基百分比在88.53%以上,数据质量符合要求。获得差异表达基因的总数为916个,其中,上调的差异基因有248个、下调的差异基因有668个。筛选出差异的转录因子属于26个转录因子家族,可能参与大豆蛋白合成的转录因子家族有4个,分别为b HLH、AP2/ERF、MYB、B3。【结论】大豆蛋白的内质网加工途径中蛋白传导通道的Sec61基因发生下调,导致蛋白合成能力下降,品种蛋白含量降低,推测Sec61基因是蛋白合成的关键基因。造成品种间蛋白差异的相关代谢途径与蛋白内质网加工、硫代谢、谷胱甘肽代谢等通路紧密相关。

【Objective】Screening transcription factors related to soybean protein synthesis and forecasting potential genes and metabolic pathways for protein synthesis.【Methods】Using RNA-Seq technology,high-throughput sequencing of soybean seeds was carried out on Zhongdou 4901,a low protein soybean material,and Huaidou 98-28,a high protein soybean material.【Results】43.14 Gb Clean Data were obtained.Each sample had a data volume greater than 6.46 Gb,a GC content greater than 45.73%,a Q20 base percentage greater than 94.78%,and a Q30 base percentage greater than 88.53%.The data quality complied with the standards.916 genes that were differentially expressed,248 of which were up-regulated and 668 were down-regulated.The screened differentially expressed transcription factors came from 26 transcription factor families,of which 4 families,b HLH,AP2/ERF,MYB,and B3,may contribute to soybean protein synthesis.【Conclusion】The Sec61 gene of protein conduction channel in the endoplasmic reticulum processing pathway of soybean protein was down regulated,resulting in the decrease of protein synthesis ability and decrease of protein content in some varieties.The Sec61 gene was thought to be the crucial gene for protein synthesis.Protein endoplasmic reticulum processing,thiometabolism,and glutathione metabolism were related metabolic mechanisms that contribute to the difference in protein content between varieties.