P16,P53及Ki67在子宫颈鳞状细胞癌(SCC),宫颈上皮内瘤变(CIN)及慢性炎症中表达水平及临床意义

Expression levels and clinical significance of P16,P53 and Ki67 in cervical squamous cell carcinoma,cervical intraepithelial neoplasia and chronic cervicitis

目的研究子宫颈鳞状细胞癌(SCC)、宫颈上皮内瘤变(CIN)及慢性炎症中P16,P53及Ki67的表达情况,并探讨其相关性。方法选取2019年10月至2021年9月于甘肃省张掖市第二人民医院行宫颈癌筛查的患者,根据纳入、排除标准最终入组193例患者,其中子宫颈SCC 5例,CIN 92例(CIN178例,CIN29例,CIN35例),慢性宫颈炎症96例,采集患者宫颈组织检测P16,P53及Ki67表达情况,并分析其与宫颈病理变化的相关性。结果慢性炎症、CIN1~CIN3以及子宫颈SCC中P16阳性率分别为4.17%、30.77%、55.56%、60.00%、80.00%,P53阳性率分别为7.29%、20.51%、44.44%、60.00%、60.00%,Ki67阳性率分别为4.17%、30.77%、55.56%、60.00%、80.00%,P16、P53、Ki67阳性率呈上升趋势,差异均有统计学意义(P<0.05);P16(r=0.462,P<0.05),P53(r=0.353,P<0.05)及Ki67(r=0.502,P<0.05)表达情况与宫颈组织病理变化正相关。结论P16,P53及Ki67在子宫颈SCC、CIN不同分级以及慢性炎症中,其表达水平随宫颈病变程度加重而升高,通过检测相关指标可评估宫颈病变情况,有利于临床预防和治疗宫颈癌。

Objective To study the expression of P16,P53 and Ki67 in cervical squamous cell carcinoma(SCC),cervical intraepithelial neoplasia(CIN)and chronic cervicitis,and explore their correlation with clinical changes.Methods People who received cervical cancer screening in the hospital from October 2019 to September 2021 were selected.According to the inclusion and exclusion criteria,193 patients were finally enrolled,including 5 patients with cervical SCC,92patients with CIN(78 CIN1,9 CIN2,5 CIN3),96 patients with chronic cervicitis.Cervical tissues were collected from different groups to detect the expression of P16,P53 and Ki67,and their correlation with cervical pathological changes was analyzed.Results P16 positive rates in chronic cervicitis,CIN1~CIN3,and cervical SCC were 4.17%,30.77%,55.56%,60.00%,and 80.00%respectively.P53 positive rates were 7.29%,20.51%,44.44%,60.00%,and 60.00%respectively.Ki67 positive rates were 4.17%,30.77%,55.56%,60.00%,and 80.00%respectively.P16 positive rates,P53 positive rates and Ki67 positive rates all showed an upward trend(P<0.05).The expression of P16(r=0.462,P<0.05),P53(r=0.353,P<0.05)and Ki67(r=0.502,P<0.05)were positively correlated with cervical pathological changes.Conclusions The expression levels of P16,P53 and Ki67 increase with the aggravation of cervical lesions.Therefore,detection of related indicators can help assess the condition of cervical lesions,which is beneficial to clinical prevention and treatment of cervical cancer..