动脉瘤性蛛网膜下腔出血患者外周血单个核细胞中瞬时受体电位通道M2水平与介入治疗预后的关系

Correlation between transient receptor potential melastatin 2 in peripheral blood mononuclear cells and the prognosis of interventional treatment in patients with aneurysmal subarachnoid hemorrhage

目的 分析动脉瘤性蛛网膜下腔出血(aSAH)患者外周血单个核细胞中瞬时受体电位通道M2(TRPM2)水平与介入治疗预后的关系。方法 选取2019年8月至2021年5月在三亚市中医院接受介入治疗的148例aSAH患者作为研究对象。用Ficoll密度梯度离心法和蛋白质免疫印迹法检测外周血单个核细胞中TRPM2水平。用改良Rankin量表(mRS)评分评价介入治疗90 d后预后。用logistic回归分析TRPM2与aSAH患者介入治疗预后的关系。结果 根据随访后m RS评分将患者分为预后良好组(n=107)和预后不良组(n=41)。预后良好组年龄、Hunt-Hess分级Ⅲ级占比、脑血管痉挛占比和Glasgow昏迷量表(GCS)评分低于预后不良组(均P<0.05)。预后良好组治疗前后TRPM2水平低于预后不良组(均P<0.05)。治疗后TRPM2>0.28和Hunt-Hess分级Ⅲ级是a SAH患者预后的独立危险因素(P<0.05)。结论 aSAH患者介入治疗后TRPM2水平高与预后不良有关。

Objective To investigate the correlation between transient receptor potential melastatin 2(TRPM2) in peripheral blood mononuclear cells and the prognosis of interventional treatment in patients with aneurysmal subarachnoid hemorrhage(aSAH). Methods A total of 148 patients with aSAH, who received interventional treatment at the Sanya Municipal Hospital of Traditional Chinese Medicine of China between August 2019 and May 2021, were prospectively collected. Ficoll density gradient separation method and protein immunoblotting method(Western blotting) were used to measure TRPM2 in peripheral blood mononuclear cells.Modified Rankin Scale(mRS) score was used to evaluate post-intervention 90-day prognosis. Logistic regression analysis was adopted to analyze the relationship of TRPM2 to the prognosis of interventional treatment.Results Based on postoperative follow-up mRS scores the patients were divided into good prognosis group(n=107) and poor prognosis group(n=41). The age, percentage of patients with Hunt-Hess grade Ⅲ, percentage of patients having cerebral vasospasm, and Glasgow coma scale(GCS) score in the good prognosis group were remarkably lower than those in the poor prognosis group(all P<0.05). Both the pre-treatment and post-treatment TRPM2 levels in the good prognosis group were significantly lower than those in the poor prognosis group(all P <0.05). Pre-treatment TRPM2 >0.28 and Hunt-Hess grade Ⅲ were the independent risk factors for prognosis in patients with a SAH(P<0.05). Conclusion In patients with aSAH after receiving interventional treatment, a high TRPM2 level is closely correlated with a poor prognosis.(J Intervent Radiol, 2022, 31:655-659)