宁神合剂联合“颐神调气”针刺法对更年期焦虑症患者神经内分泌、免疫应答及外周血miRNA表达影响

Effects of Ningshen Mixture(宁神合剂) Combined with“Yishen Tiaoqi”Acupuncture on Neuroendocrine,Immune Response and mi RNA Expression in Peripheral Blood of Climacteric Anxiety Patients

目的观察宁神合剂联合“颐神调气”针刺法对更年期焦虑症患者神经内分泌、免疫应答及外周血miRNA表达影响。方法选择2020年5月—2021年5月收治的108例更年期焦虑症患者为研究对象,以随机数字表法分组,54例对照组给予“颐神调气”针刺法治疗,54例研究组在对照组基础上给予宁神合剂治疗,治疗前、后检测两组患者雌二醇(E2)、促黄体生成素(LH)、促卵泡刺激素(FSH)、5-羟色胺(5-HT)、促性腺激素释放激素(GnRH)、肾上腺皮质酮(CORT)、肾上腺皮质激素(ACTH)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)、干扰素-γ(INF-γ)、miRNA:miR-1301、miR-663、miR-4674水平,给予患者更年期临床症状调查表(Kupperman)、贝克焦虑量表(BAI)评分,比较两组临床疗效。结果研究组LH、FSH水平低于对照组(P<0.05),研究组E2水平高于对照组(P<0.05),研究组5-HT水平高于对照组(P<0.05),研究组COR、ACTH、GnRH水平低于对照组(P<0.05),研究组IL-2、INF-γ水平低于对照组(P<0.05),研究组IL-4、IL-10水平高于对照组(P<0.05),研究组miR-4674水平高于对照组(P<0.05),研究组患者miR-1301、miR-663水平低于对照组(P<0.05),研究组患者Kupperman、BAI评分低于对照组(P<0.05),研究组总有效率高于对照组(96.30%VS 85.19%)(P<0.05)。结论宁神合剂联合“颐神调气”针刺法对更年期焦虑症患者,可调节患者神经内分泌水平,改善免疫应答,调节外周血miRNA水平,缓解患者临床症状,提升患者临床疗效。

Objective To observe the effects of Ningshen Mixture(宁神合剂)combined with“Yishen Tiaoqi”acupuncture on neuroendocrine,immune response and miRNA expression in peripheral blood of climacteric anxiety patients.Methods 108 patients with climacteric anxiety disorder treated in our hospital from May 2020 to may 2021 were selected as the research object.They were divided into groups by random number table method.54 cases in the control group were treated with“Yishen Tiaoqi”acupuncture,and 54cases in the study group were treated with Ningshen Mixture on the basis of the control group.Estradiol(E2),luteinizing hormone(LH)and Follicle stimulating hormone(FSH),serotonin(5-HT),gonadotropin releasing hormone(Gn-RH),corticosterone(CORT),adrenocortical hormone(ACTH),interleukin-2(IL-2),interleukin-4(IL-4),interleukin-10(IL-10),interference hormone-γ(INF-γ),miRNA:the levels of miR-1301,miR-663 and miR-4674 were given the scores of menopausal clinical symptom questionnaire(Kupperman)and Baker Anxiety Scale(BAI),and the clinical effects of the two groups were compared.Results The levels of LH and FSH in the study group were lower than those in the control group(P<0.05),the levels of E2 in the study group were higher than those in the control group(P<0.05),the levels of 5-HT in the study group were higher than those in the control group(P<0.05),the levels of COR,ACTH and GnRH in the study group were lower than those in the control group(P<0.05),and the levels of IL-2 and TNF-γ in the study group were lower than those inthe control group(P<0.05).The levels of IL-4 and IL-10 in the study group were higher than those in the control group(P<0.05),the levels of miR-4674 in the study group were higher than those in the control group(P<0.05),the levels of miR-1301and miR-663 in the study group were lower than those in the control group(P<0.05),and the Kupperman and BAI scores in the study group were lower than those in the control group(P<0.05).The total effective rate in the study group was higher than that in the control group(96.30%vs 85.19%)(P<0.05).Conclusion Ningshen Mixture combined with“Yishen Tiaoqi”acupuncture can regulate the neuroendocrine level,improve the immune response,regulate the level of miRNA in peripheral blood,alleviate the clinical symptoms and improve the clinical efficacy of climacteric anxiety patients.