2017-2019年河南省漯河市严重急性呼吸道感染病例人腺病毒基因特征分析

Genetic characteristics of human adenovirus in severe acute respiratory infection cases in Luohe city,Henan province from 2017 to 2019

目的了解我国河南省漯河市严重急性呼吸道感染(severe acute respiratory infection,SARI)病例人腺病毒(human adenovirus,HAdV)基因特征。方法对2017年10月至2019年2月SARI病例中鉴定的HAdV阳性标本进行病毒分离后,扩增并测定其Hexon基因的Loop2区域,初步鉴定病毒分离株型别。然后根据初筛结果,分别使用HAdV各型特异性引物扩增病毒株3个靶基因(Penton base、Hexon和Fiber)的序列全长,并与各型原型株以及国内外相应型别代表株进行系统发育和序列同源性分析,以确定HAdV病毒株型别并了解其基因特征。结果从河南省漯河市783份SARI病例中鉴定的27份HAdV阳性咽拭子标本中,共分离获得18株病毒分离株,分子分型结果显示,这些毒株分属于B亚属(HAdV-3、HAdV-7和HAdV-55)、C亚属(HAdV-1、P1H2F2、Px1/Ps3H5F5、P89H5F5和HAdV-6)和E亚属(HAdV-4)。其中C亚属HAdV-1分离阳性率最高(33.3%),其次为B亚属HAdV-3(22.2%)和C亚属P1H2F2(11.1%)。本研究4种HAdV病毒株(HAdV-3、HAdV-4、HAdV-7和HAdV-55)基因组序列在时间和空间上具有显著的保守性和稳定性特点,而HAdV-C鉴定了3种基因重组模式(P1H2F2、Px1/Ps3H5F5和P89H5F5),其潜在的公共卫生意义需进一步研究证实。结论2017—2019年河南省漯河市SARI病例HAdV感染主要以C亚属为主,其次为B亚属和E亚属,该数据为当地腺病毒相关传染病的预防控制提供了科学依据。

Objective To understand the genetic characteristics of human adenovirus(HAdV)in severe acute respiratory infection(SARI)cases in Luohe city,Henan province,China.Methods After viral isolation of HAdV-positive specimens identified in SARI cases from October 2017 to February 2019,the Loop2 region of the Hexon gene was amplified and determined to initially identify the virus type.Then,based on the preliminary screening result,the full length of the sequences of the three target genes(Penton base,Hexon and Fiber)of the viral strains were amplified using specific primers for each HAdV type,and phylogenetic and sequence homology analyses were performed with the prototype strains and the representative strains of the corresponding types at home and abroad to identify the types of viral strains and understand their genetic characteristics.Results A total of 18 viral isolates were obtained from 27 HAdV-positive throat swab specimens from 783 SARI cases in Luohe city,Henan province,and the molecular typing result showed that these strains belonged to species B(HAdV-3,HAdV-7 and HAdV-55),species C(HAdV-1,P1H2F2,Px1/Ps3H5F5,P89H5F5 and HAdV-6)and species E(HAdV-4).Among them,the highest positive detection rate was found for species C HAdV-1 isolates(33.3%),followed by species B HAdV-3(22.2%)and species C P1H2F2(11.1%).The four HAdV strains in this study(HAdV-3,HAdV-4,HAdV-7 and HAdV-55)were characterized by significantly conserved and stable in time and space;while three patterns of genetic recombination(P1H2F2,Px1/Ps3H5F5 and P89H5F5)were identified for HAdV-C strains in this study,and their potential public health significance needed to be confirmed by further studies.Conclusions The HAdV infection of SARI cases in Luohe city,Henan province during 2017-2019 was dominated by species C,followed by species B and species E.These data provided a scientific basis for the prevention and control of local adenovirus-associated infectious diseases.