北京黑猪肌内脂肪含量高、低组间脂质组差异分析

Analysis of Lipidome Difference between High and Low Intramuscular Fat Content Groups in Beijing Black Pigs

旨在揭示北京黑猪肌内脂肪含量与脂质组学间的关系。本试验主要是从400头210日龄的北京黑猪中按肌内脂肪含量高低筛选出高肌内脂肪组11个样品,其肌内脂肪含量的平均值为3.89%,低肌内脂肪组19个样品,其肌内脂肪含量的平均值为0.81%;对挑选出的高、低两组的个体取60 mg的肌肉样品进行脂质提取,最后进行非靶向脂质组学的检测;针对检测结果,对两组进行多元化分析,利用T检验、差异倍数和偏最小二乘判别分析进行标记物的筛选。结果,共检测出组成9类脂质的104种脂肪酸,其中酰基肉碱(AcCa)3个、甘油二酯(DG)4个、甘油三酯(TG)23个、心磷脂(CL)8个、磷脂酰胆碱(PC)21个、磷脂酰乙醇胺(PE)29个、磷脂酰甘油(PG)3个、磷脂酰肌醇(PI)6个、磷脂酰丝氨酸(PS)7个,通过进行T检验初步筛选(P<0.05)出33种脂质,其中TG有23个,AcCa有2个,DG有4个,PE有4个。进一步通过VIP值(VIP>1)和FC值(FC>2或FC<0.5)进行筛选,最终筛选出22个脂质,其中TG有21个,DG有1个。结果表明,脂质组学可以作为区分北京黑猪肌内脂肪含量高低的方法,构成肌内脂肪含量高低猪肉样品的脂质组差异物质主要集中在TG和DG上。

The purpose of this experiment was to reveal the relationship between the intramuscular fat content and lipidomics in Beijing black pig. In this experiment, 11 samples of high intramuscular fat group and 19 samples of low intramuscular fat group were selected from 400 210-day-old Beijing black pigs, and the average intramuscular fat content was 3.89% and 0.81%, respectively. For the selected individuals in high and low groups, 60 mg muscle samples were taken for lipid extraction, and finally non-targeted lipidomics detection was carried out. According to the test results, the two groups were analyzed by diversification, and the markers were screened by T test, difference multiple and partial least squares discriminant analysis. A total of 104 fatty acids, including 3 acylcarnitine(AcCa), 4 diglycerides(DG), 23 triglycerides(TG), 8 ardiolipin(CL), 21 phosphatidylcholine(PC), 29 phosphatidylethanolamine(PE), 3 phosphatidylglycerol(PG), 6 phosphatidylinositol(PI) and 7 phosphatidylserine(PS) were detected. The 33 kinds of lipids were preliminarily screened by T-test(P<0.05), of which TG were 23, AcCa were 2, DG were 4 and PE were 4. Further screening by VIP value(VIP>1) and FC value(FC>2 or FC<0.5),there were 22 kinds of lipids, including 21 TG and 1 DG. The results showed that lipidomics could be used as a method to distinguish the intramuscular fat content of Beijing black pig. The lipid difference substances affecting intramuscular fat content mainly concentrated in TG and DG.